Enzyme Lab Report Essay

Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area), temperature on function, reusing the catalase, reaction rate of iced liver returned to room temperature, and effects of pH on enzyme activity, to see how much O2 was released by each reaction. For experiment two they tested effect of temperature on liver, iced had a slow increase of O2 loss, after a minute 18.8 ml of O2 escaped, at 6 min the O2 escape had slowed down and O2 levels were at 20.5 ml. the room temperature lost
and boiled never released any O2 and stayed at zero. Boiled denatured so it didn’t release any O2. Liver at 37*c had a faster O2 release because it was at an optimum recapture. Room temp, had a middle range release of O2 because it wasn’t too cold, or too hot, but saturated and plateaued. Iced released little O2 because the reactions were slowed down by the cold temp (non-optimum). In experiment 3 they realized that, they couldn’t reuse the liver. They know because nothing happened, the substrate was all used leaving only liquid. Taking a new liver meant the reaction would occur with the H2O2 because it has a new substrate, and that would cause a reaction to occur. In activity 4 they tested whether a previously iced liver returned to room temperature would lose O2 faster or an iced liver would release more O2. The found that the previously iced liver lost more O2, this happened because the warmer the liver, the faster the reaction, because it was closer to an optimum temperature. our data however did no show this due to air escape from the gas collector apparatus. For experiment 5 they tested the effect of pH on enzyme activity, by giving each liver a different