Discussion Questions 1. 2. What is a spectroscope and what is it used for? A spectroscope is a scientific instrument that is used to measure the intensity of the light at different wavelengths by calculating the amount of light energy that is being absorbed by the electrons at different wavelengths. 3. 4. 6. How might the difference in the brightness of spectral lines be explained? In other words‚ why are some lines in a spectra easier to see and appear brighter? Some lines in a spectra are easier
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Main aim of experiment 5 is to use the biuret test to quantify the concentration of the Bovine Serum Albumin (BSA) then constructing a standard curve graph and to use the spectrophotometer to perform an enzyme assay using different concentration of the BSA. Experiment 5 also verifies the Beer-Lambert Law‚ which is the linear relationship between absorbance and concentration of an absorbing species. Absorbance formula is shown in fig. 1.1. However‚ the Beer-Lambert Law is not obeyed at high concentration
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Discussion of Results and Scientific Explanation: In order to observe how much a chemical substance absorbs light a spectrophotometer can be used. A spectrophotometer measures the amount of light absorbed by measuring the difference in the intensity of the light before and after a beam of light passes through a sample solution. The area in which light is absorbed can provide information about the molecule‚ such as the concentration‚ by identifying the most preferentially absorbed wavelength.1
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Available online at www.scholarsresearchlibrary.com Scholars Research Library J. Nat. Prod. Plant Resour.‚ 2011‚ 1 (1): 91-100 (http://scholarsresearchlibrary.com/archive.html) Cressa Cretica Linn: An Important Medicinal Plant-A Review on Its Traditional Uses‚ Phytochemical and Pharmacological Properties Sangeeta Rani1*‚ Sudhir Chaudhary1‚ Pradeep Singh1‚ Garima Mishra1‚ K. K. Jha1‚ R. L. Khosa2 Department of Pharmacognosy‚ Teerthanker Mahaveer College of Pharmacy‚ Teerthanker Mahaveer University
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produced by energy. When a chemical burns‚ the common atomic structures of chemicals excites the electrons and makes them go crazy. This ends up in the emission of visible light and forms into multiple colors. What is spectroscopy and how is it used by physicists and chemists? Spectroscopy is an important tool used in astronomy. It is detailed study of light that comes from an object. Light is energy that moves through space. It can be thought of either waves or particles. The distance between the high
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Nahom Assefa CHM-201 General Chemistry and Laboratory I Laboratory 8. Spectroscopy Purpose: Spectroscopy is the study of matter interacting with electro-magnetic radiation (e.g. Light). In this lab‚ we will separate some light sources in to constituent colors and find out the wavelength of some atomic emissions. We will also try to compare the line spectra found from gas discharge tubes and flame emissions. Finally‚ we will measure
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Determining absorbance of various wavelengths of light for pigments present in Coleus plants Joseph Yung (King Yung) 212831426 Adrian Ionescu Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions
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Name : Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic
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Colorimetry Introduction Absorption Spectroscopic methods of analysis rank among the most widespread and powerful tools for quantitative analysis. The use of a spectrophotometer to determine the extent of absorption of various wavelengths of visible light by a given solution is commonly known as colorimetry. This method is used to determine concentrations of various chemicals which can give colours either directly or after addition of some other chemicals. As stated by the Beer-Lambert Law
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Introduction What is Protein? There are more than 300 amino acids in the nature. However‚ only 20 amino acids are joined by each other with peptide bonds in different sequences and numbers. These sequences are formed by helping of genetic information which is encoded in genes. So‚ each protein molecule has its own amino acid sequence. Proteins are most abundant long chain macromolecules in all cells and they serve as structure and function matter in cells of all living organisms such as hormones
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