biological compatibility with a dicarboxylate pseudo crown receptor designed to satisfy the size and charge requirements of the Pb2+ cation. Spectroscopic measurements with LF1 were performed under simulated physiological conditions (20 mM HEPES‚ buffer pH 7). LF1 displays a characteristic fluorescein-like absorption band in the visible region centred at 490 nm and weak fluorescence. Upon addition of Pb2+‚ the fluorescence intensity intensity of LF1 increases by 18 fold with the absorption and emission
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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Method: The ratio of [HPO42-] to [H2PO4-] required to produce buffer solutions at pH values 5.9‚ 6.9 and 7.9 were calculated. 0.1M of H2PO4- and 0.1M HPO42- were used to mix appropriate volumes to 25mL of each of the buffer solutions. The calibrated pH meter was used to measure and record the pH of each buffer solution and then were compared to the pH of 25mL of distilled water. 3.00 mL of 0.1M NaOH was added to each of the 25mL buffer samples and to the distilled water and were mixed well in each
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First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.1 M potassium phosphate buffer at pH 6 3.4007g of potassium phosphate was weighed and placed in 300 mL beaker. Then‚ 125 mL of water was added into the beaker that contained potassium phosphate. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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LEE ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 88‚ NO. 5‚ 2005 1269 DIETARY SUPPLEMENTS Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices‚ Beverages‚ Natural Colorants‚ and Wines by the pH Differential Method: Collaborative Study JUNGMIN LEE U.S. Department of Agriculture‚ Agricultural Research Service‚ Pacific West Area (PWA)‚ Horticultural Crops Research Laboratory Worksite‚ 29603 University of Idaho Ln‚ Parma‚ ID 83660 ROBERT W. DURST and RONALD E. WROLSTAD Oregon
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the appropriate buffer solution to each. ( I will be testing pH 4‚5‚7‚8‚8.8 and 10) 3. Add 4cm³ of distilled water and 4cm³ of the appropriate buffer solution to 6 control test tubes labelled CA-CF to see if the pH alone will affect anything. 4. Place all test tubes in a water bath (40-45oC) and put the 1cm x 1cm squares of film in to them and start the stop clock. 5. From time to time‚ check if the squares have cleared and note down the times. 6. Repeat 3 times. Results pH buffer Actual pH Time for
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to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the catecholase was less effective. Also‚ when there was a higher concentration of potato juice and a lower concentration of phosphate buffer‚ absorbance of the enzyme increased. Introduction According to Edmund J. Stellwag‚ in his article "Enzyme" an
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[Accessed 22 Oct. 2014]. Inkling.com‚ (2014). Inkling. [online] Available at: https://www.inkling.com/read/marks-medical-biochemistry-lieberman-marks-4th/chapter-45/i--plasma-proteins-maintain [Accessed 13 Oct. 2014]. Media.lanecc.edu‚ (2014). Chemical Buffer Systems and Acid-Base Balance. [online] Available at: http://media.lanecc.edu/users/driscolln/RT127/Softchalk/Acid_Base_Lesson/Acid_Base_Lesson4.html [Accessed 13 Oct. 2014]. Research.vet.upenn.edu‚ (2014). Milk Urea Nitrogen. [online] Available at:
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Abstract A buffer is a solution that resists changes in pH when H+‚ OH-‚ or H20 is added. By using standard lab equipment‚ a lab pro diagnostic tool‚ and acidic and basic solutions‚ the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid. Introduction The
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First‚ the pH probes were calibrated using buffer solutions with known pH values of 4.01 ± 0.01 and 7.00 ± 0.01. First‚ the pH probes were placed in the 4.01 buffer and the probe was calibrated‚ then rinsed with distilled water‚ placed into the 7.00 buffer‚ and calibrated again. The pH probe was very close in its measurements‚ as the 4.01 ± 0.01 buffer was measured to have a pH of 4.01 ± 0.01‚ and the 7.00 ± 0.01 buffer was measured to have a pH of 6.87 ± 0.01. After the calibration
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