Preview

The Effects of Ph and Concentration Levels on Catecholase

Powerful Essays
Open Document
Open Document
1267 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
The Effects of Ph and Concentration Levels on Catecholase
Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic, the catecholase was less effective. Also, when there was a higher concentration of potato juice and a lower concentration of phosphate buffer, absorbance of the enzyme increased.

Introduction According to Edmund J. Stellwag, in his article "Enzyme" an enzyme is “a catalytic protein produced by living cells.” Enzymes function as catalysts by lowering the amount of energy required for a reaction to occur. “Enzymes already have a wide range of applications, including chemical synthesis, biodegradation of harmful chemicals, environmental and medical diagnostics, medical therapeutics, food manufacturing, detergents, and agriculture” (Kuchner). Because enzymes are biological molecules, changes in pH can greatly change their effectivity in chemical reactions. A change in the concentration of enzymes in a given area can also greatly affect the rate of reaction. The purpose of this lab was to observe and to record the activity of the enzyme catecholase in different pH levels as well as its effectivity in different concentration levels. Hypothesis A: If pH level increases (becomes more basic), then absorbance of the enzyme catecholase will increase. Null Hypothesis A: If pH levels increase, then absorbance of the enzyme catecholase will decrease. Hypothesis B: If the concentration of potato juice in an area increases and phosphate buffer decreases, then absorbance will increase. Null Hypothesis B: If the



Cited: Daniel Wellner, G. P. Royer, Edmund J. Stellwag, "Enzyme," in AccessScience, McGraw-Hill Companies, 2008, http://www.accessscience.com Neil A. Campbell, Jane B. Reece, Lisa Andrea Urry, Michael L. Cain, Steven Alexander Wasserman, Peter V. Minorsky, Robert Bradley Jackson. Biology Ninth Edition. San Francisco: Pearson, Benjamin Cummings, 2011. Print. Olga Kuchner, "Enzymes and directed molecular evolution," in AccessScience, McGraw-Hill Companies, 1998, http://www.accessscience.com

You May Also Find These Documents Helpful

  • Good Essays

    Enzyme

    • 585 Words
    • 3 Pages

    For Activity A, we first tested enzyme activity. First, we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then, we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that, we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that, we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting, we rinsed the unlabeled cup we used earlier when we tested enzyme activity. Then we used a H2O2 syringe to transfer 10 mL of H2O2 into the rinsed unlabeled cup. After five minutes, we transferred 1 mL of the boiling catalase into the unlabeled cup with H2O2 in it with an unused transfer pipet and observed the results. After testing the effect of boiling on enzyme activity, we tested for catalase in living tissue. First, we rinsed the unlabeled 60 mL cup we used earlier. Then, we used a scalpel to cut a small piece of liver. After that, we macerated the piece of liver with a glass rod. When the liver was macerated enough, we put it in a cup with 10 mL of H2O2, which was transferred into the cup with a H2O2 syringe. Lastly, we observed the cup.…

    • 585 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Nt1310 Unit 2 Lab Report

    • 1346 Words
    • 6 Pages

    2. Referring to the experiments on the effect of pH on enzyme activity, respond to the following items pertaining to those experiments: (11)…

    • 1346 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    Biology 101 Lab Paper

    • 275 Words
    • 2 Pages

    * The objective of the experiment is to assess the effect of different pH conditions on enzyme activity.…

    • 275 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Catalase Lab Report

    • 970 Words
    • 4 Pages

    6. Plug in the Go Link onto the USB drive of the computer (See Figure 1)…

    • 970 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in, but not changed by or consumed in, a chemical reaction. The amount of energy required for a reaction to take place is called the activation energy. Enzymes function by lowering that activation energy. They tend to speed up the rate of the reaction. As a result of lessening the activation energy needed and speeding up the rate of the reaction, products are formed quicker. Enzymes have a special region called the active site, where they are shaped to fit with specific molecules known as substrates. As the substrate goes into the active site, the enzyme slightly changes the shape of the substrate allowing it to form a tight fit. Binding of the substrates happen at the active site, where they are then shaped to form an enzyme-substrate complex where they are then turned to and released as products. The rate of the reaction is dependent upon the concentration of the enzyme. Enzymes are sensitive to changes in pH and temperature. These factors affect the speed of the reactions, as does enzyme concentration. The enzyme catecholase and the compound catechol are found in the cells of many fruits and vegetables. The enzyme catecholase and substrate catechol separate from each other in intact cells. However, whenever the cell is damaged, they come in contact with one another which produces the formation of benzoquinone, which is a brown substance. Benzoquinone molecules bond, forming melanin, which is what you see as the dark spots on bruised fruits and vegetables. Enzymes are sensitive to changes in H+ concentration. Being most enzymes are proteins, they have a large number of…

    • 516 Words
    • 3 Pages
    Good Essays
  • Good Essays

    If the enzyme catalase is placed in different solutions containing different pH levels, the enzyme will function most effectively at a neutral pH, or a pH of 7. This is proven through the data collected in the experiment. The reason for this is because the human body functions at a optimum pH6 when digesting lactose, which makes the pH7 in the experiment the most prime event to produce the optimum amount of substrate…

    • 537 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Many enzymes are proteins that lower the activation energy needed to start chemical reactions that are crucial to the survival of life. Alike to other proteins enzymes undergo changes in shape based on the surrounding environment, and thus their productivity changes as well. Many factors affect the productivity of enzyme including temperature, concentration of enzyme in a reaction, and pH (Gundlach 441).…

    • 1388 Words
    • 6 Pages
    Better Essays
  • Better Essays

    Cited: Reece, Jane, and Neil Campbell. Campbell Biology. 9th ed. Pearson Education Inc., 2011. Chapt 2. Print.…

    • 1105 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate.…

    • 608 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 1053 Words
    • 5 Pages

    The AP Biology classes performed this study, to examine the effects of changes in the optimum conditions for enzyme activity.…

    • 1053 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Enzymes Lab Report

    • 1657 Words
    • 7 Pages

    In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. (Unit 7: Enzymes lab)…

    • 1657 Words
    • 7 Pages
    Better Essays
  • Powerful Essays

    Before doing research on amylases, an explanation of enzymes is needed to fully understand each part of the experiment. An enzyme is a protein that helps speed up a chemical reaction (Raven, 2011). An enzyme is a catalyst, which is made by living cells; usually enzymes assist in developing the metabolic process of cells (Underkofler et al, 1958). The way in which enzymes speed up the chemical reaction is by lowering the activation…

    • 2769 Words
    • 12 Pages
    Powerful Essays
  • Good Essays

    Enzyme Lab Report

    • 418 Words
    • 2 Pages

    Enzymes are proteins that enable chemical reactions. In the enzyme lab, the effects of concentration, temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction.…

    • 418 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Enzyme Catalysis Lab

    • 1096 Words
    • 5 Pages

    Based on this experiment and the data collected one is able to conclude that the optimal environment in which this specific catalase is able to function is around 40°C…

    • 1096 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Catecholase Lab Report

    • 223 Words
    • 1 Page

    The results of the experiment falsified the alternative hypothesis that increasing temperature would increase enzymatic activity. In his study, Yagˇar concludes that the optimal temperature for catacholase is 40oC, which means that the color intensity of the solution should be greatest around 40oC (2004). Past this temperature, the color intensity should decrease. These conclusions however are not consistent with the results of the experiment. This inconsistency could be due to the excess drops of catechol in test tube “10”, and this excess reactant may have contributed to the intense color even though the solution was cooled. Another inconsistency is the intensity of test tube “50”. By Yagˇar’s results, the catecholase should have denatured,…

    • 223 Words
    • 1 Page
    Satisfactory Essays