Lab #1 Extraction and Thin Layer Chromotography
Lab #1
Extraction and Thin Layer Chromatography
Kaya Gaudet 6784928
January 9, 2013
Line Structure References
BenzophenoneBiphenylBenzoic Acid
Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the impurities behind. TLC plating can be used to mark the progress of the extraction.
Thin Layer Chromatography (TLC) Plating: This is a common method that is used to monitor the progress of a reaction and determine when it is complete. In our chromatography there was a stationary phase/adsorbant of Silica gel and we used mobile phases of ethyl acetate, hexanes, and a 2:8 mix of both. During the process, the mobile phase passes over the compound spots at the bottom and drags them up the adsorbant. The resulting spots where the compounds stop can be used to measure their polarity and solubility in the mobile phase. We had a reference spot, unknown spot, and compound spot where we mixed both.
Salting: In an extraction, if the compound is partially soluble in water then the process of salting out can help further separate it into the organic layer by increasing the polarity of the aqueous solution and forcing the organic compound out.
Procedure and observations:
Part A: Extraction of water soluble dyes (an introduction to extracting) added 1ml of ether and 1ml of distilled water into two test tubes, then added 0.006M of methylene blue to one test tube and 0.006M of methyl red solution to the other and shook each vigorously with a stopper in the first test tube there was blue on the bottom and clear on top, in the second test tube it was yellow on top and purple on bottom
Salting out added 5ml of distilled water, roughly 1-2 drops of 0.003M aqueous crystal violet
Extraction and Thin Layer Chromatography
Kaya Gaudet 6784928
January 9, 2013
Line Structure References
BenzophenoneBiphenylBenzoic Acid
Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the impurities behind. TLC plating can be used to mark the progress of the extraction.
Thin Layer Chromatography (TLC) Plating: This is a common method that is used to monitor the progress of a reaction and determine when it is complete. In our chromatography there was a stationary phase/adsorbant of Silica gel and we used mobile phases of ethyl acetate, hexanes, and a 2:8 mix of both. During the process, the mobile phase passes over the compound spots at the bottom and drags them up the adsorbant. The resulting spots where the compounds stop can be used to measure their polarity and solubility in the mobile phase. We had a reference spot, unknown spot, and compound spot where we mixed both.
Salting: In an extraction, if the compound is partially soluble in water then the process of salting out can help further separate it into the organic layer by increasing the polarity of the aqueous solution and forcing the organic compound out.
Procedure and observations:
Part A: Extraction of water soluble dyes (an introduction to extracting) added 1ml of ether and 1ml of distilled water into two test tubes, then added 0.006M of methylene blue to one test tube and 0.006M of methyl red solution to the other and shook each vigorously with a stopper in the first test tube there was blue on the bottom and clear on top, in the second test tube it was yellow on top and purple on bottom
Salting out added 5ml of distilled water, roughly 1-2 drops of 0.003M aqueous crystal violet