Preview

Effects of Sucrose Concentration On Cell Respiration In Yeast

Powerful Essays
Open Document
Open Document
2192 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Effects of Sucrose Concentration On Cell Respiration In Yeast
Effects of Sucrose Concentration On Cell Respiration In Yeast

Abstract

This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration, so we measured the rate of cellular respiration by the amount of CO2 produced per minute. The results show a trend wherein increased concentrations of sucrose increase the rate of cellular respiration.
Introduction

All living cells require energy in order to proceed with cellular processes such as active transportation, and the synthesis of molecules. ATP (Adenine Tri-Phosphate) is a molecule, which provides energy in a form that cells can use for such cellular processes. Cellular respiration is the process in which cells produce this energy to survive. It occurs in the mitochondria of the cell and is is vital for the survival of most organisms because cells cannot use the energy in glucose until it is stored in ATP. In the presence of oxygen, organisms can respire aerobically. The balanced chemical equation for aerobic respiration is: C6H12O6 + 6O2 -> 6 CO2 + 6 H2O + ~ 36-38 ATP

In the process of aerobic respiration, C6H12O6 is first broken down into 2 3-Carbon molecules called pyruvate or pyruvic acid through the process of Glycolysis, which literally means, “Sugar decomposition.” A net of 2 ATP is produced during Glycolysis. When oxygen is available, these 2 pyruvates move on to the Krebs cycle and electron transport chain to produce the remaining 34-36 ATP. Fig 1.1: An overview of Aerobic Cell Respiration (http://www.phschool.com/science/biology_place/biocoach/images/cellresp/glucover.gif)

In the process of anaerobic respiration, C6H12O6 is also broken down into 2 pyruvates through the process of Glycolysis. However because oxygen is unavailable, instead of the Krebs cycle and the electron transport system occurring, fermentation occurs

You May Also Find These Documents Helpful

  • Good Essays

    Respirationlabbackground

    • 733 Words
    • 2 Pages

    Aerobic cellular respiration is a pivotal process in which organisms carry out in order to sustain life. It is characterized by the release of energy from organic compounds by means of chemical oxidation within the mitochondria of the cell. The reactants are glucose and oxygen, and after a series of complex steps, the products of carbon dioxide, water, and ATP + heat are released. Thus, cellular respiration is an exergonic process, since heat energy is released in order to do cellular work. The overall process can be encapsulated by the following equation: C6H12O6 + CO2 6CO2+ 6H2O+ 586 kilocalories of energy/mole of glucose oxidized. This reaction seems very straightforward, however there are numerous enzyme-mediated reactions that occur within it that are not so perceptible from the simplified equation. Cellular respiration consists of three major stages: The first is Glycolysis; (occurring in the cytosol) in which chemical energy is harvested by oxidizing glucose into two 3 carbon molecules of pyruvate, and thus producing a net of 2 ATP molecules through substrate-level phosphorylation, as well as a net of 2 NADH molecules. Subsequently, the Krebs Cycle commences after 2 pyruvate molecules are converted to 2 Acetyl CoA molecules in the intermembrane space of the mitochondria. During the Krebs Cycle (occurring in the mitochondrial matrix)4 CO2 molecules are released, 1 ATP molecule is formed (for each turn of the cycle), and the reduced forms of 6 NADH and 2 FADH carry the electrons to the next step: the Electron Transport Chain. This occurs in the inner membrane of the mitochondria, and consists of many electron carriers that pass electrons (donated by NADH and FADH2) along through a series of redox reactions. At the end of the chain, oxygen acts as a final electron acceptor and it reduced them to form water. A proton motive force, or H+ gradient,…

    • 733 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Bio Lab

    • 962 Words
    • 4 Pages

    ATP is generated from aerobic respiration from the use of biosynthetic pathways. Glycolysis is where respiration starts in the cells and produces ATP, NADH, and 2 pyruvate molecules from the oxidation of six carbon carbohydrate and glucose. Even if oxygen is there or not, enzymes are mediated in the cytoplasm. The electron transport chain, chemiosmosis, and aerobic respiration use NADH molecule (which it main purpose is to transport electrons form one molecule to another) for later purposes. The mitochondrial matrix receives pyruvate from the cytoplasm after it crosses over the mitochondrial membrane. When the pyruvate enters the Krebs cycle it goes through many stages of biochemical enzyme-catalyzed reactions. In more detail about the cycle its main purpose is to produce little amounts of ATP by removing carbon dioxide and hydrogen from pyruvate molecules. Within the inner membrane of the mitochondrion the electron transport chain and chemiosmosis synthesis ATP with hydrogen ions which are NADH and FADH2. The Krebs cycle and glycolysis produce less ATP because chemiosmosis synthesizes a great amount of ATP.…

    • 962 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Cellular respiration is conducted in 3 processes: Glycolysis, Citric Acid Cycle, and Oxidative Phosphorylation. A glucose molecule enters into Glycolysis, and then is transformed to two Pyruvate molecules; it then changes again to Coenzyme A before entering into the mitochondria. Once inside the, each molecule enters the Citric Acid Cycle converted into Citrate and circulates through 3 times. The electrons expelled from this process are taken to the inner mitochondrial membrane by NADH and FADH2 carriers. They then flow through the Electronic Transport Chain, and Chemiosmosis, in a process called Oxidative Phosphorylation, which generates energy used to create ATP. This process can be broken down into the chemical formula:…

    • 1062 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The Citric Acid Cycle is a series of enzyme-catalysed reactions that take place in the mitochondrial matrix of all aerobic organisms. It involves the oxidation of the acetyl group of acetyl CoA to two molecules of carbon dioxide. Each cycle produces one molecule of ATP by substrate-level phosphorylation, and reduces three molecules of NAD and one molecule of FAD for use in Oxidative Phosphorylation. The cycle is preceded by Glycolysis, which also occurs in anaerobic respiration, and the pyruvate dehydrogenase complex, which occur in the cytoplasm and the mitochondrial matrix respectively. In aerobic respiration, glycolysis breaks down one molecule of glucose and two molecules of pyruvate, and gives a net product…

    • 1383 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    To be able to carry on metabolic processes in the cell, cells need energy. The cells can obtain their energy in different ways but the most efficient way of harvesting stored food in the cell is through cellular respiration. Cellular respiration is a catabolic pathway, which breaks down large molecules to smaller molecules, produces an energy rich molecule known as ATP (Adenosine Triphosphate) and a waste product that is released as CO2. Basically, cellular respiration is a metabolic process that releases energy from organic compounds (such as C6H12O6) by metabolic chemical oxidation in the mitochondria within each cell. Proteins, carbohydrates, and fats can all be broken down into fuel for the cell but cellular respiration is usually correlated with glucose. Cellular respiration also requires O2 to carry out its pathway, as oxygen will act as a final electron acceptor. So, the final equation that can be represented for Cellular Respiration is: C6H12O6 + 6 O2 → 6 CO2 + 6 H2O + heat.…

    • 1940 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    Atp Energy System

    • 969 Words
    • 4 Pages

    * In Aerobic glycolysis, glucose or glycogen is broken down to pyruvic acid via glycolitic enzymes during carbohydrate metabolism. Hydrogen is released and glucose is metabolized to pyruvic acid. In the presence of oxygen, the pyruvic acid is converted into acetyl coenzyme A. (Acetyl CoA). 1 mole of glucose produce 2 moles of ATP or 1 mole of glycogen produces 3 moles of ATP.…

    • 969 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Photosynthesis Lab

    • 310 Words
    • 2 Pages

    In this lab, we will investigate the effect of sucrose concentration on the rate of cellular respiration in yeast. Under specific conditions, yeast will convert sucrose into glucose and then use this glucose in cellular respiration.…

    • 310 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Cell Work Sheet

    • 850 Words
    • 4 Pages

    A living version of internal combustion-is the main way that chemical energy is harvested from food and converted to ATP energy, it is also called an aerobic process, which is just another way of saying that it requires oxygen. So cellular respiration is defined as the aerobic harvesting of chemical energy from organic fuel molecules. The three stages are; glycolysis, the citric acid cycle, and the electron transport.…

    • 850 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Glucose, or any carbon-based molecule, can be burned in oxygen (oxidized) to produce carbon dioxide and water. Combustion reactions release large amounts of energy. However, the energy release is uncontrolled. An organism would not be able to handle all that energy at once to do the work of the cell. Cellular respiration is essentially the same reaction as combustion, but the oxidation of glucose occurs in several controlled steps. The same amount of energy is ultimately released, but it is gradually released in small, controlled amounts. High potential energy molecules of ATP are produced while the carbon atoms are used to form various other molecules of lower potential energy. Each of these steps is catalyzed by an enzyme specific to that step. Model 1 illustrates the ideal circumstances for cellular respiration. In some situations, however, one glucose molecule may not result in 38 ATP molecules being…

    • 1212 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Unit two Biology

    • 7492 Words
    • 30 Pages

    The substance that is used to produce ATP in a cell by respiration is known as a respiratory substrate. So far, we have described respiration as if the only respiratory substrate was glucose. In fact, many cells in the body are able to use other substances as respiratory substrates, especially lipids and proteins. (Brain cells are unusual in that they can use only glucose.) Figure 2.15 shows the metabolic pathways by which glucose is oxidised in aerobic respiration.…

    • 7492 Words
    • 30 Pages
    Powerful Essays
  • Good Essays

    Cellular Respiration Lab

    • 452 Words
    • 2 Pages

    The main objective of this lab is to see if the rate of cellular respiration will be affected if we change the food source from glucose to three different experimental variables (fructose, sucrose, lactose). Cellular Respiration is a process that generates ATP and it involves the complete breakdown of glucose to carbon dioxide and water. Carbohydrates, fats, and proteins can all be used as fuels in cellular respiration, but glucose is most commonly used as an example to examine the reactions and pathways involved. Cellular Respiration can be divided into three metabolic processes; Glycolysis that occurs in the cytoplasm, Krebs cycle that takes place in the matrix of the mitochondria, and Oxidative phosphorylation that occurs via the electron…

    • 452 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Glycolysis Process

    • 379 Words
    • 2 Pages

    Cellular respiration, in the process of ATP synthesis, refers collectively to intercellular reactions in which energy-rich molecules are broken down to form ATP, using O2 and producing CO2 (2). In most cells, ATP is generated from the disassembling of absorbed nutrient molecules in three stages, glycolysis: in the cytosol, the citric acid cycle: in the mitochondrial matrix and oxidative phosphorylation: at the mitochondrial inner membrane (1). The 6-carbon molecule of Glucose is broken down into 3-carbon pyruvate molecules during the nine stages of glycolysis. However, for this reaction to occur, 2 ATP molecules must be broken down to power the segregation process of glucose into 2 pyruvates (3). Throughout this process, glycolysis produces four ATP, which results in an overall gain of ATP for the entire…

    • 379 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Cell Energy

    • 1057 Words
    • 5 Pages

    The main function of cellular respiration is to generate ATP for cellular work; it is the process of harvesting chemical energy from organic fuel and converting it to ATP energy. The three stages of cell respiration include: Glycolysis, Citric Acid Cycle, and Electron Transport(Simon, Reece, & Dickery, 2010).…

    • 1057 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Aerobic cellular respiration is the release of energy from organic compound from organic compounds by metabolic chemical oxidation in the mitochondria within each cell. Cellular respiration involves a series of enzyme-mediated reactions. The equation below shows the complete oxidation of glucose. Oxygen is required for this energy-releasing process to occur.…

    • 1687 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    Cellular Energetics is the broad term that encompasses both cellular respiration and photosynthesis and refers to how energy changes and reacts within cells. Cellular respiration is the process by which cells break down sugars (ATP) in order to produce energy for other chemical reactions. Cellular respiration takes place mainly in the mitochondria and the reactants in this process are oxygen and glucose and the main product in this process is ATP as well as waste products which include carbon dioxide and water. Almost all organisms perform cellular respiration. There are two types of cellular respiration…

    • 1719 Words
    • 7 Pages
    Better Essays