Experiment on Cellular respiration of Mung Beans Seeds with the Effects of Temperature

Introduction: ATP is generated from aerobic respiration from the use of biosynthetic pathways. Glycolysis is where respiration starts in the cells and produces ATP, NADH, and 2 pyruvate molecules from the oxidation of six carbon carbohydrate and glucose. Even if oxygen is there or not, enzymes are mediated in the cytoplasm. The electron transport chain, chemiosmosis, and aerobic respiration use NADH molecule (which it main purpose is to transport electrons form one molecule to another) for later purposes. The mitochondrial matrix receives pyruvate from the cytoplasm after it crosses over the mitochondrial membrane. When the pyruvate enters the Krebs cycle it goes through many stages of biochemical enzyme-catalyzed reactions. In more detail about the cycle its main purpose is to produce little amounts of ATP by removing carbon dioxide and hydrogen from pyruvate molecules. Within the inner membrane of the mitochondrion the electron transport chain and chemiosmosis synthesis ATP with hydrogen ions which are NADH and FADH2. The Krebs cycle and glycolysis produce less ATP because chemiosmosis synthesizes a great amount of ATP.
In the electron transport chain there is a final hydrogen acceptor which is oxygen in an aerobic respiration. A by-product of this respiration is oxygen which is used to form H2O. All the reactants that are needed for this reaction are oxygen, substrates of phosphorylation, and glucose. This will produce ATP, water, and carbon dioxide. To find respiration in a organism people may measure the amount of carbon dioxide released, the amount of glucose used, or the amount of oxygen consumed. The experiment will be to incubate the mung beans seeds in one of two treatments of temperatures, measure respiration, and take down data of the amount of oxygen taken in by the seeds during the incubation time.
Hypothesis: If the temperature changes and the



Cited: Danyk, H. (2012). Biology 1010 Laboratory Manual Fall, 2012. Lethbridge, Alberta: Department of Biological Sciences . Pechenik, J. A. (2013). A short guide to writing about biology (8th ed.). New York, NY: HarperCollins College Publishers.

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