A Study of Cellular Respiration & Gas Production in Peas
We conducted this lab in order to determine the relationship between gas production and the cellular respiration rate between germinating peas and dormant peas. Cellular respiration is the procedure of changing the chemical energy of organic molecules into a form of energy that can be used by cells called ATP (adenosine tri-phosphate). Cellular respiration is conducted in 3 processes: Glycolysis, Citric Acid Cycle, and Oxidative Phosphorylation. A glucose molecule enters into Glycolysis, and then is transformed to two Pyruvate molecules; it then changes again to Coenzyme A before entering into the mitochondria. Once inside the, each molecule enters the Citric Acid Cycle converted into Citrate and circulates through 3 times. The electrons expelled from this process are taken to the inner mitochondrial membrane by NADH and FADH2 carriers. They then flow through the Electronic Transport Chain, and Chemiosmosis, in a process called Oxidative Phosphorylation, which generates energy used to create ATP. This process can be broken down into the chemical formula: C6H12O6 + 6O2 6CO2 + 6 H2O + ATP
In this lab we compare the effects of adding heat and cold to the germinating peas, dormant peas, and glass beads to find how this changes their cellular respiration rate. The effects are measured by testing the amount of CO2 released from each sample with different temperatures applied. This is important to learn the difference between the rate of respiration during dormancy and germination. Hypothesis
My hypothesis for this experiment is that the germinating peas that have heat applied will give off the most CO2 because they are more active and heat speeds up the process by weakening molecular bonds. I believe the non-germinating peas applied to cold will give the lowest respiration rate because they are not as active and cold slows the cellular respiration process down. The room temperature and the...
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