bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar
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iLab #2 Carbohydrate Tests INTRODUCTION - For this experiment we will be testing four different bacteria with four different tests‚ using glucose‚ lactose‚ and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case
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Medical Microbiology II - Laboratory Report 1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral
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(2001). Group A streptococcalphagocytosis resistance is independent ofcomplement factor H and factor H-like protein 1binding. Mol. Microbiol. 41‚ 817–826 Nizet V McCoy‚ A. J.‚ Liu‚ H.‚ Falla‚ T. J.‚ and Gunn‚ J. S. (2001).Identification of Proteus mirabilis mutants with increasedsensitivity to antimicrobial peptides.Antimicrob.AgentsChemother.45‚ 2030–2037. Peschel‚ A. (2002). How do bacteria resist humanantimicrobial peptides? Trends Microbiol10‚ 179–186. Peschel‚ A.‚ Otto‚ M.‚ Jack‚ R. W.‚ Kalbacher
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3. Results and discussion 3.1 Nutritional analysis In the present study‚ the potential benefits were shown by nutritional attributes of dried bathua (Table1). Moisture content and dry matter analysis‚ reporting during nutritional analysis is very important because it directly affects the nutritional content of vegetable. The moisture content was quite low (5.06%) which may be advantageous in view of increasing the sample’s shelf life. Bathua was found to be rich in carbohydrates (40.84%). There
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between 4 and 11 depending on the species. The optimum temperature also varies by species. History Catalase was first noticed in 1811 when Louis Jacques Thénard‚ who discovered H2O2 (hydrogen peroxide)‚ suggested its breakdown is caused by an unknown substance. In 1900‚ Oscar Loew was the first to give it the name catalase‚ and found it in many plants and animals. In 1937 catalase from beef liver was crystallised by James B. Sumner and Alexander Dounce and the molecular weight was worked out
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Hydrolysis Report Bile Esculin Hydrolysis‚ Starch Hydrolysis‚ UreaHydrolysis‚ Casein Hydrolysis Hydrolysis Report Introduction Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis‚ hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell‚ it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell‚ it
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Introduction: Catheter-Associated Urinary Tract Infections (CAUTIs) are one of the most costly occurring nosocomial infections seen today. The Center for Disease Control (CDC) (2015) stated that the risk of developing CAUTIs is 3%-7% in the acute care setting‚ and CAUTIs comprise 40% of all institutionally acquired infections with an 8% prevalence rate seen in the home care setting. The CDC (2015) further implied that more than 1 million patients who either resides in an acute-care hospital or extended-care
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I
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