Identification of Neisseria and Moraxella Species Lab Report

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  • Topic: Neisseria, Gram negative bacteria, Neisseria meningitidis
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  • Published : April 2, 2012
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Medical Microbiology II - Laboratory Report 1

Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis, ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans, iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used, iv. recognize gram-negative diplococcic in urethral and cervical smears, and, v. identify: Neisseria meningitides, Neisseria gonorrhoeae, Neisseria lactamica, and Moraxella catarhalis.

Method: Lab manual page(s) 27-8

Results:
Part A) A, B, C, D unknown samples

Colonial appearance:
|Samples |Colonial appearance | |A |Cinnamony colour with small round, mucoid colonies. | |B |Resembles (A). Yellow- tan colour, round, smooth edges. | |C |Smaller colonies, grayish-white, smooth, shiny and semi-transparent | |D |Greyish white colonies, irregular (smooth), opaque and dry. |

Gram stain microscopic morphology:
|Unknowns |Gram reaction and microscopic morphology | |A |Gram- diplococci, with slight pigmentation. | |B |Gram- diplococci | |C |Gram- diplococci | |D |Gram- diplococci |

Identification tests:
|Organism |Oxidase |Catalase | Glucose | Maltose | Lactose (OPNG)| Sucrose |DNase | |A |+ |+ |+ |+ |+ |+ |- | |B |+ |+ |+ |+ |- |- |- | |C |+ |+ |+ |- |- |- |- | |D |+ |+ |- |- |- |- |+ |

API strip (D sample):

|Test |Results | |PENicillinase |Blue (neg) | |GLUucose |Yellow (pos) | |FRUctose |Red (neg) | |MALtose |Red (neg) | |SACcharose/Sucrose |Red (neg) | |Ornithin DeCarboxylase |Grey-green (neg) | |UREase |Pink-violet (neg) | |LIPase |Colorless (pos) | |ALcaline Phosphatase |Colorless (neg) | |Beta GALastosidase |Colorless (neg) | |Proline Arylamidase |Pale orange (neg) | |Gamma Glutamyl Transferase |Yellow (neg) | |INDol...
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