To compare the amount and type of microbial growth on areas we would normally consider clean to the amount of microbial growth on an item we would consider dirty. Methods and Materials:
Materials: Agar Plate, unopened gum wrapper, handicap button to open door to the women’s restroom, compound microscope, slide, Bunsen burner, inoculating loop, crystal violet, iodine, alcohol, safranin, and distilled water. Steps:
1. rub one finger on gum wrapper and wipe on agar plate for the clean side 2. rub a different finger on handicap button for bathroom and wipe on agar plate for dirty side 3. place in incubator to allow microbial growth for 24 hours 4. after the 24 hours, make a smear for the clean side and the dirty side on individual slides 5. perform a gram stain on each slide and view under microscope Results:
For my clean side results, I saw gram positive bacilli.
Gram Positive Bacilli (clean side)
Gram Negative and gram positive staphylococci, gram positive cocci
For my dirty results, I saw a combination of gram negative staphylococci, gram positive cocci, and gram positive staphylococci.
In this lab I discovered that what we really consider clean is never really “clean”. My un-opened gum wrapper seemed almost just as dirty as the handicap button for the women’s restroom. It makes me realize that there are bacteria and different microbes absolutely everywhere, and even in places we would never think they would be.