Study Guide

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Study guide for Microbiology Chapter 2

1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text.

2. Define / describe each of the following as they apply to microbiology: a. Culturef. pure culture
b. Inoculumg. contaminated culture
c. Inoculationh. mixed culture
d. Colony

3. Microbiologists employee a number of approached to acquiring a pure culture from a from sample containing a number of different types of bacteria. Briefly describe three different procedures commonly used to secure pure cultures from a mixed culture. The use of simple labeled diagrams may be quite helpful.

4. Microbiological media exists in a variety of forms and are classified on the basis of certain criteria. a. Describe the various physical states into which media are commonly classified

b. Describe the various groups into which media can be classified on the basis of chemical makeup.

c. List the various groups into which media can be classified on the basis of their respective functions.

5. Which method is used to check the movement/ motility of bacteria?

6. Fill in the table below with a brief, concise description of the functions of each of the types of media, respectively.

|Growth Medium |Function | | | | | | | | | | | | | | | | | | | | | | | | |

7. Distinguish between the resolving power and magnifying power of a microscope lens system.
a. resolving power:

b. magnifying power:

8. In addition to bright field microscopy list two other variations on the optical microscope.

9. Why is it necessary to use immersion oil when using the 1000X objective lens to observe a specimen?

10. Complete the following table of units of measure commonly used in microbiology.

|Unit |Abbreviation |Exponential Value | |1 centimeter |cm | | | |mm |10-3 | | |μm |10-6 | |1 nanometer |nm | | |1 angstrom |Å | |

11. Describe the procedure for making a bacterial smear for microscopic observation...
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