Bios242 Lab2
iLab #2
Carbohydrate Tests
INTRODUCTION -
For this experiment we will be testing four different bacteria with four different tests, using glucose, lactose, and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case scenario and identify the bacteria, then check to see if our guess was correct. The findings are that we were able to identify, by process of elimination, the four different test bacteria.
PROCEDURE -
These procedures were followed for all four media test samples.
Label test sample.
Record Case study and Gram Stain results.
For glucose testing:
Select red glucose broth with durem tube.
Inoculate red glucose media.
Incubate for 24 hours.
Record results.
For lactose testing:
Select red lactose broth.
Inoculate red lactose media.
Incubate for 24 hours.
Record results.
For sucrose testing:
Select red sucrose broth.
Inoculate red sucrose media.
Incubate for 24 hours.
Record results.
For Identifying the four organisms:
View identification matrix and elimination hierarchy.
Make an educated guess with information from case study.
Identify organism.
Verify and record the correct bacterium.
OBSERVATIONS & RESULTS -
|Acid from glucose |Gas from glucose |Lactose ferm. |Sucrose ferm. | |Test bacterium 1 |+ |+ |+ |+ | |Test bacterium 2 |+ |+ |+ |+ | |Test bacterium 3 |+ |- |- |- | |Test bacterium 4 |+ |+ |- |+ | |
Test bacterium 1 - Escherichia coli
case study: A 4-year old girl was brought to the emergency room suffering from severe, watery diarrhea, lethargy, and nosebleeds. Emergency room personnel reported a fever of 101o F, blood pressure of 110/70, and heart rate of 72. Blood and stool samples were taken.
Carbohydrate Tests
INTRODUCTION -
For this experiment we will be testing four different bacteria with four different tests, using glucose, lactose, and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case scenario and identify the bacteria, then check to see if our guess was correct. The findings are that we were able to identify, by process of elimination, the four different test bacteria.
PROCEDURE -
These procedures were followed for all four media test samples.
Label test sample.
Record Case study and Gram Stain results.
For glucose testing:
Select red glucose broth with durem tube.
Inoculate red glucose media.
Incubate for 24 hours.
Record results.
For lactose testing:
Select red lactose broth.
Inoculate red lactose media.
Incubate for 24 hours.
Record results.
For sucrose testing:
Select red sucrose broth.
Inoculate red sucrose media.
Incubate for 24 hours.
Record results.
For Identifying the four organisms:
View identification matrix and elimination hierarchy.
Make an educated guess with information from case study.
Identify organism.
Verify and record the correct bacterium.
OBSERVATIONS & RESULTS -
|Acid from glucose |Gas from glucose |Lactose ferm. |Sucrose ferm. | |Test bacterium 1 |+ |+ |+ |+ | |Test bacterium 2 |+ |+ |+ |+ | |Test bacterium 3 |+ |- |- |- | |Test bacterium 4 |+ |+ |- |+ | |
Test bacterium 1 - Escherichia coli
case study: A 4-year old girl was brought to the emergency room suffering from severe, watery diarrhea, lethargy, and nosebleeds. Emergency room personnel reported a fever of 101o F, blood pressure of 110/70, and heart rate of 72. Blood and stool samples were taken.