25922 + red Proteus mirabilis 12453 + colourless Salmonella typhimurium 14028 + colourless Streptococcus faecalis 29212 - or partial Uses Acting as a visual ph indicator‚ the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-). This medium is also known as an "indicator medium" and a "low selective medium". Absence of electrolytes serves to inhibit swarming by Proteus species. Lac+
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OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree of Bachelor of Science in Biotechnology in Masinde Muliro University of Science and
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Required materials include stock cultures of organisms to be tested. Methicillin resistant S. aureus‚ Streptococcus pyogenes‚ Streptococcus pneumoniae‚ Proteus mirabilis‚ and ESBL K. pneumoniae would be excellent choices because of their characteristic morphologies. (Gladwin & Trattler 2011) Multiple Cefepime and Ceftriaxone antibiotic discs will be needed to preform sensitivities. 5% sheep blood agar plates
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MEDICALLY IMPORTANT BACTERIA | GRAM-POSITIVE BACTERIA * Alpha and gamma hemolysis on blood agar * Bacillus cereus colonies on blood agar * Beta-hemolysis on blood agar (S.aureus) * Beta-hemolysis on blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain
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Multiplication of contaminant bacteria in urine and interpretation of delayed culture. Abstract A prospective study of the bacterial populations of non-infected urine was mounted in an attempt to define the length of delay between voiding and analysis during which culture would not give false positive results due to the multiplication of contaminant bacteria present at the time of voiding. The findings suggest that culture of urine within four hours of voiding is likely to give a true indication
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maggot therapy mechanisms of action Medicinal maggots have at least two confirmed beneficial effects on wounds that can be supported by laboratory investigations. They are debridement‚ or elimination of necrotic tissue‚ and removal of pathogenic bacteria. There is moreover‚ evidence from recent studies that they may also accelerate wound healing by promoting the formation of granulation tissue as suggested by the early literature‚ Wound debridement activity Necrophagous larvae feed on the dead
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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(28%) ethanolic extract propolis (EEP) of [Bazian‚ Pshdar‚ Sharbazher‚ Khormal‚ Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus‚ Staphylococcus epidermidis‚ Escherichia coli‚ Pseudomonas aerugenosa‚ Klebsiella pneumoniae‚ Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus
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Microbiology Sec 147848 November 18‚ 2013 Title of the Experiment: Enterobacteriaceae Identification: The Enterotube II System Learning Objectives: After completing this exercise we were able to inoculate an unknown bacterium that belongs to the Enterobacteriaceae by using technology effectively with a Enterotube II. An Enterotube II is a miniaturized multi-test system for rapid identification of enterbacteriaceae. We then evaluated the test results and generated a five-digit code for the unknown
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