Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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Increasing Fiber in Rice Pilaf Arcelay‚ Marilyn March 30‚ 2007 FOS 4041L Dr. Dixon Introduction Obesity is a word that everyone is currently familiar with. The media and health professionals have been working tirelessly to make the general public aware of its prevalence and detriments to society. With the staggering statistics of 32.2% prevalence in adults and a range of 13.9% to 18.9% prevalence in children and adolescents‚ these outstanding numbers stand
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artists‚ was pursuing his pet theory--that his own nasal mucus had antibacterial effects--when he left a culture plate smeared with Staphylococcus bacteria on his lab bench while he went on a two-week holiday. When he returned‚ he noticed a clear halo surrounding the yellow-green growth of a mold that had accidentally contaminated the plate. Unknown to him‚ a spore of a rare variant called Penicillium notatum had drifted in from a mycology lab one floor below. Luck would have it that Fleming had
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine- used
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test
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Unit 6 Lab Report 1. Objective: To find the percent composition of water in an unknown hydrate. 2. Materials: Goggles‚ apron‚ evaporating dish‚ hot plate‚ and stirring rod 3. Procedure: get the mass of a evaporating dish‚ put a sample of the hydrate onto the evaporating dish and mass the dish with hydrate. Put the dish with hydrate on the hot plate on high heat. Break up any clumps that form during the heating process. Observe any color
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Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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Introduction The purpose of this experiment is to see how much bacteria can and will grow in common places in a typical high school. Common places can mean many things‚ including anything from water fountain spouts to computer keyboards. Such objects can hold up to 2‚700‚000 colony forming units per square inch (or CFU/in sq)(NSF). A colony forming unit is the unit used to find an estimate of the number of cells of a bacteria. This unit of measurement is commonly utilized in the subject of microbiology
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