1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
Premium Erlenmeyer flask Laboratory glassware Water
Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
Free Bacteria Staining Microbiology
GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
Premium Gram staining Bacteria Staining
The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
Premium Gram staining Staining Microbiology
1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
Free Bacteria Staining Microbiology
GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
Premium Gram staining Staining
Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
Premium Gram staining Bacteria Staining
did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the
Premium Escherichia coli Bacteria Agar plate
Laboratory(TFMB1001) Report Experiment Tittle Gram stain Experiment Date 24October2014 Submission Date 31October2014 Students Name Tittle Gram stain. Objectives Aim of this experiment is to differentiate between the two major categories of bacteria ‚ gram positive and gram negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink
Free Bacteria Staining Gram staining
Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different
Premium Bacteria Microbiology Gram staining