Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10‚ 37‚ 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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(Justification-2 Enzyme Inhibition) By quantitative balance‚ the total amount of Enzyme is [E] 0= [E] + [EI] + [ES] + [ESI]. By using a=1+[I]/KI and a′=1+[I]/K′I‚ it is followed by [E]0=[E]a+[ES]a′ This equation can be written like this‚ [E]0=(Km[ES])/([S]0)a + [ES]a′=[ES]( aKm/[S}0+a’)‚ because of Km=[E][S]/[ES] and [S]≈[S]0. V=kb [ES] =kb [E] 0/ (aKm/[s] 0+a’). Kb [E] 0 is Vmax. This is why V=Vmax/(a^’+aKm/[S]0). This equation can be rearranged like this‚ 1/V= a’/Vmax+(aKm/Vmax)1/[S]0‚ which is
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being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to bring the solution into a required volume for gel electrophoresis.
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as
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How Enzymes work in the home and in industry essay This essay aims to explore the ways on how enzymes are used in home and in industry‚ and it aims to explain the advantages and disadvantages of using enzymes in the home and industry. An enzyme is a protein that is formed by the body that acts as a catalyst to cause a certain desired reaction. Enzymes are very specific. Each enzyme is designed to initiate a specific response with a specific result. Firstly‚ the AQA Science Biology textbook published
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are made up of proteins which
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Headline: Abnormal Liver Enzymes: What Does It Mean? Keywords: Liver‚ increase‚ enzymes‚ symptoms‚ test Page Description: Elevated liver enzyme is a condition in which there is an increase in the leakage of chemicals i.e. liver enzymes through liver cells. This hike leads to symptoms of various health problems. Blood test can be used in determining the associated causes and symptoms. Text: { What is meant by Elevated Liver Enzymes? Elevated liver enzymes are a common problem. Inflammation or
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