spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink Microscope Clothes pin Bunsen burner Methods: 1. Obtain your slide and draw two circles where your bacteria will go. 2. Connect you Bunsen burner and run your inoculation loop through the flame
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during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon by two microbiologists‚ Ziehl and Neelsen. Like Ehrlich‚ Ziehl and Neelsen’s procedure requires heat to force the primary dye through the cell wall. Another microbiologist‚ Kinyoun‚ developed a
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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Companion Program. Journal of Community Practice‚ 15(3)‚ 77-98. doi:10.1300/J125v15n0305 Collins‚ W. L. (2011). Culturally Competnt Practices: Working with Older African Americans in Rural Communities. Social Work & Christianity‚ 38(2)‚ 201-217. Dye‚ C. J.‚ Willoughby‚ D. F.‚ & Battisto‚ D. G. (2011). Advice from Rural Elders: What it Takes to Age in Place. Educational Gerontology‚ 37(1)‚ 74-93. doi:10.1080/03601277.2010.515889 Feist‚ H.‚ Parker‚ K.‚ Howard‚ N.‚ & Hugo‚ G. (2012). New Technologies:
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MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained it is called positive staining. It
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using different temperatures. c. fossil fuels ______4. It consist of paraffin wax‚ beeswax mixed with coal tar-dye. d. foot powder ______5. The protein where sulfanilamide was synthesized e. fractional distillation ______6. It is a preparation of soap‚ tragacanth‚ and glycerin to soften the skin f. chemotherapy ______7 . Compounds which fasten dyes g. lanolin ______8. Wool grease from sheep h. mordants ______9. Study of fighting diseases-causing
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02 II (2) | (a) The figure below shows the initial set-up of a model of the mass flow hypothesis for translocation in the phloem.Model of mass flow hypothesis (i) It was observed that the dye moved from osmometer X to osmometer Y during the course of the experiment. Explain this observation according to the mass flow hypothesis. (3 marks) (ii) Explain the function of tubing M in this model. In plants‚ which tissue serves the same function as tubing M? (3
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Osmosis is the movement of water across a membrane. It always navigates to the area of the membrane with a higher solute concentration. We take a closer look at the effects of osmosis in this lab through the examination of red blood cells (sheep)‚ plant cells (elodea)‚ and active transport in yeast. Under the microscope‚ we can determine the effects on plant and animal cells exposed to hypotonic‚ hypertonic‚ and isotonic sodium chloride solutions. Plant cells have a cell wall; however‚ animal cells
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test‚ the objective was to find the test materials that have a reducing sugar. In the reducing sugar test benedicts was used. In the grease spot test‚ the objective was to find all the samples that had a lipid. Another one of the lipid tests were the dye test which was a more specific test to find the substances that all contained a fat by using a solution of Sudan 4 and water. The protein test’s purpose was to determine all the test materials that contained a protein by using biuret solution. The procedure
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The largest dispersion of Jews was in the 8th century before the common era‚ however most of the Jews in Split came during the 3rd-4th century Romaniote immigration‚ along with the Syrian and Iranian exiles. This formed a strong Jewish presence on the East Adriatic Coast. The East Adriatic Coast was a smart place to settle because of its location for economic trade‚ not to mention its mild climate and beautiful geography. Salona‚ Dalmatia‚ and Split are all prime examples of the settlement of Jews
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