example. Because the DNA is invisible‚ the loading buf fer also contains two dy e s : bromophenol blue (a small dye molecule that behaves like a DNA fragment about 600 bases long) and xylene cyanol (a larger dye that acts like a DNA fragment of about 4000 bases). These dyes form lines that give you an idea of how far your DNA has moved. Some loading buffers also have a third dye‚ behaving like a very small DNA molecule (50 bases or so). As the DNA migrates‚ the different fragments will form
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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FILM STRUCTURE FILM STRUCTURE What is motion picture film? The American National Standards Institute (ANSI) describes it as “a thick flexible strip of plastic‚ complying with a dimensional standard as defined within‚ whose use is specific to the process of manufacturing a motion picture.” That definition leads to about a dozen pages of further definitions about various aspects of motion picture film. For our purposes‚ let’s take a look at how film is made‚ and how an image is formed on that film. THE
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The Phoenicians were among the greatest traders of their time and owed much of their prosperity to trade. At first‚ they traded mainly with the Greeks‚ trading wood‚ salves‚ glass and powdered Tyrian purple. Tyrian Purple was a violet-purple dye used by the Greek elite to color garments. In fact‚ the wordPhoenician derives from the Ancient Greek wordphoinios meaning "purple". As trading and colonizing spread over the Mediterranean‚ Phoenicians and Greeks seemed to have unconsciously split that sea
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PRACTICAL 7 TITLE : DETERMINATION OF ERYTHROSINE CONCENTRATION USING UV- VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry
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------------------------------------------------- Chemistry It is a synthetic dye produced using aromatic hydrocarbons from petroleum.[1] It can be combined with tartrazine (E102) to produce various shades of green. It is usually a disodium salt. The diammonium salt has CAS number [2650-18-2]. Calcium and potassium salts are also permitted. It can also appear as an aluminium lake. The chemical formation is C37H34N2Na2O9S3. The dye is poorly absorbed from the gastro-intestinal tract and 95% of the ingested dye can be found in the feces. It also
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necessary as Lab Paq provided pre-diluted chemicals. Utilizing the 96-well plate‚ 2 pipit drops of each chemical were added to the wells in the following combinations: a) NaHCO3+HCl b) HCl+Bromthymol blue c) NH3+1 drop Bromthymol blue d) HCl+blue dye e) Blue dye+NaOCl followed by HCl f) NaOCl+KI followed by various test foods g) KI+Pb(NO3)2 h) NaOH+phenolphthalein i) HCl + phenolphthalein j) NaOH+AgNO3 k) AgNO3+NH3 l) NH3+CuSO4. Along the way‚ observations were made pertaining to the reactions witnessed
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Particle in a Box Lab March 25‚ 2014 Kassandra Brady‚ Samantha Imler‚ & Michael Montone CHEM 353 Abstract: Introduction: Method I: Absorption Spectra of Conjugated Dyes This method analyzes a series of dyes with alternating double bonds. It is assumed that the π electrons are free to move and ignoring the electro static repulsion among the π electrons themselves. The theoretical model applied in this experiment is the particle in the box. The model states that a single particle moves free
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Introduction & Background According to H. J. Croxx‚ when microscopists first began to use stains in the sixties and seventies‚ the demand for dyes for this purpose was naturally too small to justify a special source of supply. They therefore had to make use of textile dyes‚ which were then very crude and were not constant in their composition. Plant-based dyes such as wood‚ indigo‚ saffron‚ and madder were raised commercially and were important trade goods in the economies of Asia and Europe. Across
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gram-positive bacteria will retain the crystal violet-iodine complex. Gram-negative bacteria will not retain the color and the decolorizer will wash out the stain. F. What is the purpose of safranin in the Gram’s stain procedure? This is a basic dye that directly stains the gram-negative bacteria that have become decolorized. The gram-positive bacteria are already stained and
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