Preview

Spit Lab

Powerful Essays
Open Document
Open Document
2637 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Spit Lab
10/29/10
Honors Biology
Period 12
Chemical Aspects of Life & Spit Lab
ABSTRACT:
The objective for the Spit lab was to test two different types of crackers for the presence or absence of starch and of reducing sugars. Also, to test the chewed cracker, the one that didn’t have a reducing sugar, for the presence or absence of a reducing sugar with the saliva in it. Adding on, another part of the objective is to determine the effect of amylase on starch. For the Chemical Aspects lab, the objectives were to test for the presence or absence of carbohydrates, lipids and proteins in several test samples. In the starch test, the objective was to find the substances that contain starch. In the starch test, iodine was used. In the reducing sugar test, the objective was to find the test materials that have a reducing sugar. In the reducing sugar test benedicts was used. In the grease spot test, the objective was to find all the samples that had a lipid. Another one of the lipid tests were the dye test which was a more specific test to find the substances that all contained a fat by using a solution of Sudan 4 and water. The protein test’s purpose was to determine all the test materials that contained a protein by using biuret solution. The procedure for the Spit lab was to perform the starch test and reducing sugar test on both crackers. Then chew up the cracker that doesn’t have reducing sugars. Next, test the sample for a reducing sugar. The results of the Chemical Aspects lab were that starch, potato juice, cracker solution, and cereal contain starch. In the reducing sugar test glucose, fructose, lactose, and milk contain a reducing sugar. In the grease spot test, salad oil, milk, and chips are lipids. In the dye test, salad oil and chips are fats. Egg albumin, cereal, and cracker mixture show that they are proteins. In the Spit lab the saliva and cracker test showed up positive. The starch test for saltine was positive. The reducing sugar test for saltine was

You May Also Find These Documents Helpful

  • Good Essays

    Sludge Lab

    • 821 Words
    • 4 Pages

    A few weeks ago, I got a container full of “sludge”. I was asked to separate it into its four components and identify each one to help me better understand how to separate mixtures ad identify substances. My original sludge had a liquid on top and solid(s) on the bottom. The liquid was clear, and most of the solid was tan. There was a small layer of blackish/brownish particles in the middle of the tan solid. There were small yellow “rocks” visible on the surface of the tan solid. For the first physical observation, I tried smelling the mixture. It smelled like permanent marker. Next I tried sticking a spoon in it and stirring the tan solid lightly. It looked like it was an…

    • 821 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    lab 24

    • 332 Words
    • 2 Pages

    Purpose: The purpose of this lab is to perform several general tests which help in identifying fats, protein, and carbohydrates in food.…

    • 332 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Corn and Milk Lab

    • 290 Words
    • 2 Pages

    If I test milk for nutrients then it will test possitive for lipids,protein,and fats but negative for starch because it consists of those nutrients.…

    • 290 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Bio Lab

    • 549 Words
    • 3 Pages

    In part II of the Organic Compounds in Foods lab, we to examining whether or not the starch in the saltine cracker can chemically break down in sugar with the presence of salivary amylase, which is an enzyme found in your mouth which assists in digestion. My group and I were instructed to obtain a saltine cracker from our teacher and chew on it for approximately 2 minutes. After concluding the chewing, we then grabbed a beaker and spit our slimy and chewed cracker into it. Subsequently, to get results my group and I placed the beakers in the water bath where it heated for 3-5 minutes. We needed to put it in the water bath because for best results enzymes must be in hot temperatures and water.…

    • 549 Words
    • 3 Pages
    Satisfactory Essays
  • Powerful Essays

    Lab

    • 1313 Words
    • 4 Pages

    the average length of 14.5 mm in our control group and the average length of .47 mm…

    • 1313 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Saliva Lab

    • 1101 Words
    • 7 Pages

    Since the amylase enzyme is present in saliva, starch digestion begins in the mouth. Salivary…

    • 1101 Words
    • 7 Pages
    Good Essays
  • Better Essays

    Amylase is found in the saliva of humans and is responsible for the first step in breaking down starch. Starch is a polysaccharide composed of a large number glucose monomers joined together. Amylase breaks down starch by separating the glucose molecules into maltose, which is a two glucose-unit compound. (Morgan, 2008) During this experiment we investigated the influence of pH on the activity of the enzyme Amylase. We are able to use the fact that when I2KI is placed in a starch solution the solution turns a dark purple which shows that the starch has not been digested. When solution remains a yellow amber color this means that all the starch has been digested. Amylase was placed in five separate test tubes with each test tube having a different pH level. Using a test plate we placed a drop of the amylase solution on top of a drop of I2KI every 10-seconds to test the time it takes amylase to digest the starch. The optimal pH for amylase is determined by the shortest time of starch digestion. The pH of the saliva in the mouth ranges from 5.76- 7.96 depending on the person. (Larsen, 1998) If saliva pH ranges from 5.76 to 7.96 we believe that amylase activity will be most active between 6 to 7 pH, so the tests tubes with a pH level of 6 and 7 should have the…

    • 1386 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    PEX 08 01

    • 518 Words
    • 6 Pages

    Exercise 8: Chemical and Physical Processes of Digestion: Activity 1: Assessing Starch Digestion by Salivary Amylase Lab…

    • 518 Words
    • 6 Pages
    Satisfactory Essays
  • Good Essays

    Digestive System Lab

    • 778 Words
    • 4 Pages

    In the first part of the lab, we prepared a salivary amylase solution and aggregated acetic acid to detect the presence of mucin. Then, 4 test tubes were made of the following: Tube 1 = 3 ml starch + water + 37 degrees water bath; Tube 2 = 3 ml starch + saliva in water bath; Tube 3 = 3 ml starch (cooled) + 3 ml saliva (cooled) in ice bath; Tube 4 = 3 ml starch + 3 ml saliva 5 drops conc. HCL in water bath. An incubation period of 1 hour was followed and each was tested for starch and maltose. A similar procedure was repeated with pepsin, with the test tubes prepared as follows: Tube 1 = 5 ml pepsin 5% soln + 5 ml HCL (0.5)%; Tube 2 = 5 ml pepsin (5% soln)+ water 5 ml; Tube 3 = 5 ml HCL (0.5%) + 5 ml of water; Tube 4 = 5 ml pepsin 5% soln) + 5 ml NAOH (0.5%). Lastly, the above procedure was repeated with…

    • 778 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Lab Exer

    • 425 Words
    • 3 Pages

    Median: 50% of the households have an income more than 560,961.5883 while the other 50% have an income of less than 560,961.5883.…

    • 425 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    LAB

    • 1235 Words
    • 11 Pages

    Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is:…

    • 1235 Words
    • 11 Pages
    Better Essays
  • Good Essays

    Saliva that contains salivary amylase enzyme, which breaks down the glycosidic linkage between glucose that found in starch and hydrolyzes starch into glucose at optimum temperature of 37°C, that is similar to human body temperature and show positive results to Benedict’s test The salivary amylase enzyme is denatured at 95°C as the enzyme lost in structure. In the experiment, test tube 1 and 4 is added with saliva and brown-orange solution with precipitate is present by test tube 1 at temperature of 37oC in the first 5th minutes as it is the optimum temperature for amylase enzyme to work. For test tube 4, orange solution is formed. After the incubation period, the amount of precipitate that present at the bottom of test tube 1 increases as more starch is hydrolyzed and green-blue solution is formed at test tube 4.…

    • 511 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    The study will make use of: Agar Plates, specifically Nutrient Agar, for different tests and as a controlled variable for the problem, Nutrient Broth, same purpose as the nutrient agar, Santol (Sandoricum koet jape) extract, Escherichia coli, for the gram positive, Bacillus , for gram negative, and Candida________ a test subject for the study. Also, the study will make use of litmus paper for measuring and maintining the pH level of the Agar plates. The study will also make use of spectrophotometry.…

    • 971 Words
    • 4 Pages
    Powerful Essays
  • Powerful Essays

    Nutrients in Food

    • 1404 Words
    • 6 Pages

    Food contains nutrients that are very significant to the body. The basic essential nutrients, vitamins, and minerals play very significant functions in the different metabolic processes. In order for the nutrients to be known, several tests were performed to qualitatively analyze nutrients for the different food samples. Reagents were used as indicators to test common food substances for the presence of specific nutrients. Fruit juice, raw egg yolk, peanuts tested negative for mineral matter; margarine, raw egg yolk, peanuts tested positive for the presence of fat; fruit juice, raw egg yolk, peanuts, cooked rice, tested positive for carbohydrates; raw egg white, peanuts, cooked rice, anchovy, tested positive for the presence of proteins.…

    • 1404 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Lab

    • 451 Words
    • 2 Pages

    Introduction Water plays a vital role in biological systems. Some species are able to survive in diverse environment such as fishes in salt water, Cactus plants in dry land and mammalian cells in aqueous surrounding because of the specialized underlying structures of these life-forms. In order for us to appreciate these special adaptation, we first need to know how a typical plant or an animal cell organelle behaves in different water and solute concentrations. In this lab, we will determine the effects of hypertonic, isotonic and hypotonic solutions on plant and animal cells. In general when an animals cell's placed in hypertonic solution it shrivels; a plant cell on the other hand undergoes plasmolysis. When an animal cell's placed in hypotonic solution, it begins to swell and then finally lyse; a plant cell placed in this type of solution however, is said to be turgid. In isotonic solution, the animal cell stays the same (normal) whereas the plant cell becomes flaccid. In this lab, we started out with observing the effects of 3 different sugar solutions on Rhoeo discolor leaves. Then we observed rabbit blood cells placed in hypertonic, hypotonic and isotonic solutions. And finally, we observed some of the organelles, particularly the nucleus' of chicken cells as they were placed in detergent and water. Our results reported below show, when plant cells were placed in 0.2M, 0.3M, and 0.4M, and observed after 1 hour, the cells in high molar (0.4) solution plasmolyzed, and in low molar (0.2) solution looked normal. The rabbit cells shrunk when they were placed in hypertonic solution, and they appeared to be larger in size when placed in hypotonic solution. The chicken cells placed in detergent and also in water, appeared to have a reduction in the organelles.…

    • 451 Words
    • 2 Pages
    Good Essays