The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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#5: Dissolved Oxygen Lab Be sure to read and understand the below instructions BEFORE the lab! Experiment 1: Investigating the Effect of Temperature and Salinity on the Concentration of Dissolved Oxygen in Water Introduction: In an aquatic environment‚ oxygen must be in a solution in a free state (O2) before it is available for use by organisms (bio-available). Its concentration and distribution in the aquatic environment are directly dependent on chemical and physical factors and are greatly
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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The primary challenge of inhabiting a high salinity environment is balancing osmotic pressure. Since these environments contain high salt concentrations‚ water from the cells of organisms spontaneously diffuses out of the cytoplasm in order to restore osmotic balance. This leaves cells dehydrated and thus‚ eventually causes cell death. In order to ameliorate this predicament‚ halophiles use one of two unique strategies that function to increase the osmolarity of the cell‚ both of which as illustrated
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region of higher concentration to a region of lower concentration through a cell membrane or other semi-permeable membrane until an equilibrium is reached. It is a special case of diffusion (passive transport). Basic Explanation : Osmosis can occur when there is a partially permeable membrane‚ such as a cell membrane. When a cell is submerged in water‚ the water molecules pass through the cell membrane from an area of low solute concentration to high solute
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Lab activity 1: Effect of temperature‚ concentration‚ and pressure on equilibrium Introduction Our ongoing discussion has been on systems at dynamic equilibrium: for a reversible reaction‚ the rate of the forward reaction is equal to the rate of the reverse reaction. What happens if equilibrium is disturbed? In this lab activity‚ we are going to examine the effect of changing reaction conditions on the position of equilibrium. Part I: Effect of temperature We will consider the equilibrium
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Year 12 Nutrition Stage 2 SACE: Practical – Manipulation of Microbial Growth | August 25 2013 Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small
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Nutritional Factors that Influence Microbial Growth To live/reproduce‚ all living things need: An energy source A source of Carbon Food Sunlight – phototroph Iron etc- chemotroph If reduced form of chemical‚ organism can use it as energy Organic-chemoorganotroph Inorganic – chemolithotroph Organic cmpds- heterotroph CO2- autotrophs Nitrogen fixation- make into organic form that humans can use N is often a limiting nutrient in the environment Meaning
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DISCUSSIONS ----------------------------------------------------------- 7 * CONCLUSION ----------------------------------------------------------- 8 * REFERENCE ----------------------------------------------------------- 9 TITLE Osmotic pressure within red blood cell. INTRODUCTION Osmosis is a passive movement of water molecules going across the partially permeable membrane. It is a very spontaneous process due to the downhill energy flow known as “water potential” by which‚ water
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