The effect of environmental factors such as temperature‚ osmotic pressure‚ oxygen concentration and pH on microbial growth and survival Abstract: Microbial growth can be affected by different environmental factors such as temperature‚ osmotic pressure‚ oxygen concentration and pH. Six experiments were carried out in this report testing for microbial growth against different environmental factors. Good aseptic techniques were used to prevent contamination‚ resulting in a uniform set of results
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Effect of Osmotic Stress and Temperature on Microbial Growth BIO 3400-002L – Microbiology Lab 1 Effect of Osmotic Stress and Temperature on Microbial Growth Luiz Felipe Isidoro ABSTRACT Evolution allowed primitive forms of life to develop proteins and enzymes that made it possible for life to evolve under environments with hostile conditions‚ such as high salt and heat. More specifically‚ some bacteria selected genes that code for peptides with stronger intermolecular forces‚ coping
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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pH and Microbial Growth Introduction: Each organism has an optimum environment for growth‚ which includes pH level. Organisms that grow at near neutral pH levels are called neutrophiles. Those that grow best in acidic pH values are called acidophiles. Others‚ yet‚ grow best in alkaline pH values and are called alkaliphiles. In this lab‚ each group was assigned an organism‚ which was then used to inoculate 6 different pH levels. Materials and Methods: Followed lab procedure 29‚ “pH and
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Water moves from an area of LOW concentration of Solute to an area of HIGH concentration of solute through a semi-or fully permeable membrane by the process. Water moves constantly through the cell ’s membrane its estimated that about 250 times the volume of a single cell moves through 1 per second This continues until the solute concentration reaches equilibrium It is convenient to express the available energy per unit volume in terms of "osmotic pressure". It is customary to express this
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of presentation learner will be able * Introduction of control of microbial growth * Define term related to control of microbial growth * Describe physical method of microbial growth * Control of Microbial Growth Control of Microbial Growth: Introduction * Early civilizations practiced salting‚ smoking‚ pickling‚ drying‚ and exposure of food and clothing to sunlight to control microbial growth. * Use of spices in cooking was to mask taste of spoiled food. Some
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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OBJECTIVE: To describe the effect of water gain or loss in animal and plant cells. INTRODUCTION: Osmosis is the tendency of water to flow from a hypotonic solution (low concertration of dissolved substances) to hypertonic solution ( higher concerntration of dissolved substances) across a semipermeable membrane. Osmosis occurs when different concentrations of water are separated by a differentially permeable membrane. One example of a differentially permeable membrane
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homeostasis‚ and their action is coordinated by the hypothalamus. In Module 2 we saw how the breathing and heart rates were maintained (N.B. Synoptic questions probable!). Here we shall look at three more examples of homeostasis in detail: • temperature‚ • blood glucose and • blood water. All homeostatic mechanisms use negative feedback to maintain a constant value (called the set point). This is the most important point in this topic! Negative feedback means that whenever a change
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