THE EXTRACTION OF DNA THE EXTRACTION OF DNA 07/02/2013 | By Laura Green‚ Tasnim Rahman and Sandra Strammiello | Table of Contents DNA………………………………………………………………………………………..………… 2 The Discovery of DNA…………………………………………………………………………...…………..…………. 4 The Steps for Extracting DNA…………………………………………………………………………………………….…..… 5 Experiment: Which Product Can the Most Visible Amount of DNA Be Extracted From?..............................................................................................
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A. Title – Acid-Base Extraction with Separatory Funnel B. Introduction – The main objective of the experiment was the extract an acid‚ a base‚ and a neutral compound from a 2:1:1 compound of benzoic acid‚ p-nitroaniline‚ and azobenzene. In theory‚ an extraction technique based on phase distribution should allow two immiscible solvents to separate with a portion of the solute distributed into each. Using a separatory funnel‚ acids were extracted with bases (NaOH)‚ and bases were extracted
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Separating a Mixture of Compounds Exp. No. 2 name Chemistry 145 February 2‚ 2014 I. Purpose The purpose of this experiment was to separate this mixture into components‚ determine the mass and percentage of each component. In addition to test the accuracy of an alternative method of subtraction to find the mass of the sodium chloride directly. Start with a mixture of sand (SiO2)‚ sodium chloride (NaCl)‚ and ammonium chloride (NH4Cl)‚ and use these separation processes: sublimation filtration
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Subject:Experiment # 2: Extraction and Evaporation. Separating the Components of “Panacetin” Introduction: The problem proposed in the experiment is an unknown ingredient found in generic Panacetin tablets that must be discovered. Panacetin tablets are known to contain aspirin‚ acetaminophen‚ and sucrose; therefore‚ the tablets tested‚ containing aspirin and sucrose‚ are thought to contain an unknown of something similar to that of acetaminophen such as acetanilide or phenacetin. Another problem
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necessary reaction schemes. The reaction schemes should include molecular weights and any other important physical data for the reagents and products. Ex. Cyclohexanol was oxidized to cyclohexanone using sodium dichromate in the presence of sulfuric acid. * Note: you can download a free version of a structure editor from ACD labs‚ the PC version is called chemsketch‚ the Mac version is called marvinsketch. You should learn how to draw structures using these programs. Lab reports should look professional
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Objective: The objective of this experiment is to use acid-base extraction techniques to separate a mixture of organic compounds based on acidity and/or basicity. After the three compounds are separated we will recover them into their salt forms and then purify them by recrystallization and identify them by their melting points. Procedure: Extraction of Carboxylic Acid A pre-weighed (0.315g) mixture of Carboxylic acid‚ a phenol‚ and neutral substance was placed into a reaction tube (tube 1).
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Acid-Base Extraction: Separation of an Organic Acid‚ Base and a Neural Compound Introduction/Background Acid base extraction is a widely used commercial method in separating acids‚ bases and neutral compounds. This is concept is based on several physical properties of the compounds‚ such as boiling point‚ melting points and solubility. The different solubility properties of the protonated and non-protonated forms of these compounds depend on the functional groups present‚ such
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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Separating a Mixture of Compounds Exp. No. 2 April 8‚ 2014 I. Purpose The purpose of this experiment is to separate three components; SiO₂‚ NaCl‚ and NH₄Cl through Sublimation‚ filtration and evaporation to determine the mass and mass percentage of each component. II. Experimental Method Equipment Chemicals Evaporating Dish Powdered Mixture A 2 50ml beaker Water Balance Thermometer Bunsen burner III. Raw Data and Calculations Mass of empty evaporating dish 76.00g
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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