DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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Collaboration Component Form Please complete this four-part guide‚ and submit for the collaboration component. Make sure to save this file with the module number (no periods) and your name. Thank you. 1. Collaboration lesson/task description: Describe the lesson or task you completed collaboratively in a paragraph consisting of five or more sentences. or more sentence ‚ tons of details‚ excellent grammar‚ punctuation and spelling 2. Peer and self-evaluation: Rate each member of the team
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PUNNETT SQUARE/EXPLANATION Punnett Square F f f Ff ff f Ff ff Because this disorder is a dominant autosomal‚ the unaffected woman would have a recessive allele. Crossing the two‚ these would be the results. They had a child that would be FH‚ as descripted. ANIMATION NOTES Restriction enzymes bind/target to specific DNA sequences As they bind to the DNA sequence‚ the restriction enzymes cut the sugar-phosphate backbones of the DNA EcoRi is a restriction enzyme EcoRI cuts unevenly
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organisms) is used when we refer to crop plants using different molecular biology techniques or methods to change their properties‚ such as to enhance their resistance to herbicides‚ improve nutritional content‚ and other properties. This seems great‚ but why there are people who disapprove the development and consumption of GM foods? Modifying plant genetics can have many benefits‚ because of the use of precise and accurate molecular techniques‚ the main difference between traditional breeding techniques
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Permeability Simple Diffusion The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? NONE 1. According to your results‚ which solute had the highest molecular weight? Albumin 2. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? Na+Cl 3. Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. The larger
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Activity 1: 1. Did any solutes move through the 20 MWCO membrane? Why or Why not? 2. Did Na+Cl- move through the 50 MWCO membrane? Activity 2: 1. Are the solutes moving with or against their concentration gradient in facilitated diffusion? 2. What happened to the rate of facilitated diffusion when the number of carrier proteins was increased? 3. In the simulation you added Na+Cl- to test its effect on glucose diffusion. Explain why there was no effect. Activity 3: 1. Which membrane resulted
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Transgenic Animals: Ethical Issues and Benefits to Human Welfare Nowadays‚ breakthroughs in molecular biology are happening at an unprecedented rate. One of them is the ability to engineer transgenic animals‚ i.e.‚ animals that carry genes from other species. The technology has already produced transgenic animals such as mice‚ rats‚ rabbits‚ pigs‚ sheep‚ and cows. Although there are many ethical issues surrounding transgenesis‚ this article focuses on the basics of the technology and its applications
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Nanotechnology Applications Nanotechnology is science that involves working with matter at the molecular or nano-scale (25‚ 400‚ 000 nanometers equal 1 inch) dimension‚ the understanding and control of matter at dimensions between 1 and 100 nanometers (AmericanElements‚ 2010). Today where unique phenomena enable novel applications‚ this includes Fuel Cell Development‚ Biotechnology‚ and Medicine among others. Using the heating properties of iron oxide nanoparticles in gene regulation‚ cancer therapy
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Lab 1: Introduction to Science Exercise 1: Data Interpretation Table 4: Water Quality vs. Fish Population Dissolved Oxygen (ppm) 0 2 4 6 8 10 12 14 16 18 Number of Fish Observed 0 1 3 10 12 13 15 10 12 13 Questions 1. What patterns do you observe based on the information in Table 4? 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water. 3. What would your experimental approach be to
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Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) Definition Definition In molecular biology‚ restriction fragment length polymorphism‚ or RFLP is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites‚ and to a related laboratory technique by which these segments can be illustrated. In RFLP analysis
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