[LIVMFAP] - [GAD] [2-8]. The heme is used to oxidize drugs by adding a hydroxyl group to these substances‚ often as a way to rid the body of potentially harmful toxins in order to make them more water-soluble. They do this by inserting one atom of molecular dioxygen into an organic substrate (RH) while the second oxygen atom is reduced to water (RH+02 + 2e- → ROH + H20). CYP catalysis substrate binding to the heme site requires a two-electron transfer with NADH-cytochrome P450 oxidoreductase (CPR) to
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crystal (Methylene blue‚ solid or Potassium Permanganate KMnO4-purple) will move further than the other due to the driving force (kinetic energy)? My prediction is that Potassium Permanganate KMnO4- purple (molecular mass- 158.03 g/mol) will move further than Methylene blue‚ solid (molecular mass- 319.85 g/mol) since it weighs less. In osmosis is diffusion of H2O across a selectively permeable membrane (dialysis tubing). There is only movement of water‚ not solute‚ across the plasma membrane. Water
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Identification of Mystery Plasmids Abstract Plasmids serve as important tools in genetics and biotechnology labs‚ where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size‚ antibiotic resistance‚ lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be
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MWCO membrane. A possible reason to why none of the of these solutes were able to pass through was because the solutes could have been too big to be transported. According to your results‚ which solute had the highest molecular weight? The solute that had the highest molecular weight was Albumin. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? The solute that displayed the highest rate of diffusion through the 200 MWCO
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Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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EXAM QUESTIONS 1) Describe the alternative fates of pyruvate in cellular respiration. 2) Write notes on the structure and significance of α and β glycosidic bonds. 3) Describe the mechanism of DNA duplication. Include a brief account of the types of proteins‚ cofactors and enzymes involved. 4) Write an account of two of the key historical experiments that identified DNA as the carrier of the genetic code. 5) Describe the “central dogma” for the genetic code and the basis by which genetic information
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3D Printing: Market Research Report Phone: +44 20 8123 2220 Fax: +44 207 900 3970 office@marketpublishers.com http://marketpublishers.com Phone: +44 20 8123 2220 http://marketpublishers.com 3D Printing: Market Research Report Date: Pages: Price: ID: April 1‚ 2013 225 US$ 4‚950.00 3CBC5D8B98FEN This report analyzes the worldwide markets for 3D Printing in US$ Million by the following Product Segments: 3D Printing Products‚ and 3D Printing Services. Global market for 3D Printing is also analyzed
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Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify our foods to increase flavor‚ yield‚ nutritional value or shelf-life • Better understand human genetics • Clone cells or organs Molecular Biology’s Best Friends: Bacteria Why use bacteria? • They’re relatively simple organisms. • They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell). • It’s pretty easy
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