LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
Premium Gram staining Staining Bacteria
Purpose: To use indicators to test for the presence of organic compounds in certain substances. Hypothesis: Honey will contain sugars‚ egg white contains proteins‚ corn oil contains lipids‚ oats contains starches and proteins‚ gelatin contains sugars and proteins‚ potatoes contain starch‚ and apple juice contains lipids and sugars. Materials: 9 test tubes Test tube rack Test tube holder Grease pencil Hot plate 20 ml honey solution 20 ml egg white and water mixture 20
Premium Carbohydrate Protein Starch
producing a form of stilbene (Figure 1). This reaction favored a crude Z-Stilbene crystal product over its E counterpart. When Z-Stilbene underwent photoisomerization with iodine for 1 hour it reconfigured almost exclusively into its more stable counterpart E-Stilbene. The reaction produced very low yield of 6.3% due to the nature of the reaction and the speed at which iodine reacts. The purity of E-Stilbene could have been increased by allowing the reaction to perform longer and to use a faster reactant
Premium Chemistry Ethanol Chemical reaction
_____________________________________________ Project 3: Identifying the Unknown White Compound by Testing Physical and Chemical Properties _____________________________________________ Michigan State University Chemistry Lab 161 Section 27 19 October 2017 Friday 11:00-1:50 Introduction For this experiment‚ there was an unknown white compound that had to be identified. By testing this compound for different properties it made making an educated guess easier to determine
Premium Chemistry Chemical substance Ester
by the emergence of Shiga toxin-producing Escherichia coli (Callaway et al.‚ 2013). Specifically‚ Shiga toxin-producing E. coli O157 is a foodborne pathogen of significant public health importance. It can cause mild to bloody diarrhea in humans which may progress to hemolytic uremic syndrome (Centers for Disease Control and Prevention‚ 1993; Hussein‚ 2007) that can be fatal in children‚ the elderly and immunocompromised individuals. E. coli O157 is also responsible for an estimated 63‚153 illnesses
Premium Bacteria Escherichia coli Microbiology
providers work more efficiently. One of these medical advancements would be a mutated E. coli. So how exactly can a mutated E. coli be an advancement? Well what scientist recently discovered is that this certain mutated bacteria actually will color urine to help diagnose medical diseases. So perhaps this mutated E. coli can make diagnosing certain issues a quicker process than before. One disease this E. coli mutant helps diagnose is diabetes. To elaborate on how this altered bacteria works
Premium Diabetes mellitus Insulin Blood sugar
An Ode to E. Coli There is a natural human tendency to dismiss what we cannot see. This idea is based in evolutionary biology. Throughout most of human history‚ threats to our survival have been deadly predators . It is only natural then‚ that we should focus our concern on objects whose importance we can see. For this reason bacteria seem insignificant on the surface‚ its invisibility marking its lack of precedence as a threat. This is a misconception‚ because bacteria hold enormous power. It can
Premium Bacteria DNA Gene
Title: E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation
Premium DNA Plasmid Gene expression
Student Lab Section 6 E. Coli Genetic Transformation with pGLO Plasmid Introduction: Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). For this experiment we used the bacteria E. Coli to take in foreign jellyfish DNA which will allow it to change genetic material. This experiment determines the effects that the plasmid pGLO has in transferring the Green Florescent Protein found in a jellyfish into the bacteria. It
Premium Escherichia coli Plasmid Bacteria
Staphylococcus epidermidis is one of the most prevalent microorganisms found on the human skin and in the mucous membranes‚ however‚ it is a typically overlooked bacterium because there is very little that is known about it. Though it is not as aggressive as its cousin Staphylococcus aureus‚ Staphylococcus epidermidis is the most frequent cause for nosocomial pathogens‚ especially among newborns‚ the elderly‚ and anyone who has a compromised immune system. These infections are usually associated
Premium Bacteria Immune system Staphylococcus