data on the growth of Escherichia coli (E. coli) and to monitor how it grows under certain conditions. It has been demonstrated that the levels of glucose and dissolved oxygen were found to affect the rate of growth of E. coli proportionally with a lack of oxygen resulting in the lowering of the pH. In this experiment the growth of E. coli was studied at constant temperature (37 0C) at which it grows ideally. Experimental results for the growth of Escherichia coli showed good agreement with theory
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Erica Osorio 5057497 Christian Roque and Rogerlio The Mechanisms by which E.Coli Cells Developed Immunities toward Ampicillin due to Plasmid and DNA Consumption U34 Abstract During the ampicillin experiment the ability to transform cells to make them adaptable to their environment was studied. The E.coli bacterial cell was used in order to observe how its DNA was able to change and develop immunity towards ampicillin. In order for this change to occur the use of several
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Staphylococcus aureus: From a Spot to the Hospital The hospital is silent this morning‚ as the sun breaks through the blinds and casts off all the silver objects in the room. My hospital bed is uncomfortable‚ and it makes me yearn for my 100-year-old mattress and many unnecessary plush-pillows. The smell of bleach and cleanliness lingers in my nose and almost makes me feel sicker. How did I end up here? Well‚ that is not an adrenaline rushed injury or a freak accident‚ but something that started
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Methicillin Resistant Staphylococcus Aureus Staphylococcus aureus bacteria‚ commonly known as staph‚ are very common. It is "a spherical gram-positive parasitic bacterium of the genus Staphylococcus‚ usually occurring in grapelike clusters and causing boils‚ septicemia‚ and other infections." In fact‚ many people have some living on their skin all of the time and it can cause infection anywhere in the body. There are antibiotics that have been known to kill this bacterium. Unfortunately
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Methicillin-resistant Staphylococcus aureus September 22‚ 2013 Methicillin-resistant Staphylococcus aureus Methicillin-resistant Staphylococcus aureus also known as MRSA‚ is a bacteria that is resistant to many antibiotics. In the community‚ most MRSA infections are skin infections. In the medical facilities‚ MRSA causes life-threatening bloodstream infections‚ pneumonia and surgical site infections. (Methecillin-resistant Staphylococcus aureus (MRSA) infections‚ 2013) Individuals would
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The problems that arise regarding the antibiotic resistance of certain bacteria has continue to worry the health industries. One of these bacteria is the Staphylococcus aureus. It has been one of the major causes of hospital-acquired infections which has resulted to a lot of deaths each year. Description Staphylococcus aureus is a gram-positive bacteria which indicates that it has a cell wall structure with a thick layer of peptidoglycan. It is a facultative anaerobe which allows it to perform
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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