Pre-lab 1: Identifying Carbohydrates Abstract The point of this lab is to determine if a substance contains carbohydrates such as a reducing sugar and/or polysaccharides. This will be done by using Benedict’s reagent and Iodine stain tests. Benedict’s reagent will react to reducing substances in the solution by oxidizing it and changing the structure of the reducing sugar to form a colored precipitate. The color of this precipitate can be used to determine the concentration of reducing sugars in
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BIOLOGY LAB REPORT PRACTICAL M1 TESTING FOR MACROMOLECULES Title : Testing for Macromolecules Abstract : To test for macromolecules (carbohydrates‚ lipids‚ nucleic acids‚ and proteins)‚ iodine solution‚ alcohol‚ aceto-orcein stain and copper sulfate solution are used. The conclusions for all of the tests are positive. Introduction: A) Carbohydrate (starch) Carbohydrates are chemical compounds that contain oxygen‚ hydrogen‚ and carbon atoms. They consist of monosaccharide
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Hydrolysis of macromolecules Abstract: This lab was designed to teach the process of “hydrolysis”‚ a chemical reaction in which water is added to a polymer‚ breaking its bonds and forming smaller molecules. A hydrogen cation and a hydroxide anion (which once formed water) break apart and attach themselves to the ends of shorter polymers. Hydrolysis plays an important role in our lives and in the lives of every living thing on earth. Living organisms rely on digestion (hydrolysis) to convert food energy
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Deoxyribonucleic acid (DNA) is the fundamental molecule of life encoding the genetic code for the development and functioning of every living organism and a large variety of viruses. RNA‚ proteins‚ and DNA are the main macromolecules‚ which are necessary for every form of life. The genetic system is encoded in the form of a sequence of nucleotides (guanine‚ adenine‚ thymine‚ and cytosine)‚ which are denoted by the letters G‚ A‚ T‚ and C. Most DNA molecules consist of double-stranded helices‚ composed
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changeDried upNo change 4.sucrose solutionNo changeNo changeDried upNo change 5.starch solutionNo changeDark blue-blackDried upNo change 6.distilled waterNo change (Clear blue)No change (Clear yellow)Dried upNo change Investigation 1A: Testing for Macromolecules Discussion: By observing the table above‚ we could see that Benedict’s test was for reducing sugars‚ iodine test was for the presence of starch‚ filter paper was for the presence of fatty acids‚ and the Biuret test was for amino groups present
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Macromolecules of Life Exercise 1: Testing for Proteins In this experiment Biuret Color is added to four substances‚ egg white‚ pepsin‚ sugar‚ and distilled water to test the detection of protein in each substance. Substance Tested Predicted Results Biuret Color & Number of drops added 1. Egg White Change color 5 drops –turned a yellow/light brown 2. Pepsin Change color 5 drops- turned purple 3. Sugar No Change 5 drops-no change in color 4. Distilled Water No Change 5 drops-no
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TESTING FOR MACROMOLECULES Describe the positive test for starch. Explain how you know. The positive test for starch is adding iodine solution. In the experiment‚ the starch solution had the most dramatic reaction with the iodine solution. It turned to an opaque bluish-purple solution while other samples all turned to a colour related to orange and yellow other than the vegetable oil. But the vegetable oil was more translucent than completely opaque. Describe the positive test for
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Macromolecule Report Monomer units of biological macromolecules have heads and tails. When they polymerize in a head-to-tail fashion‚ the resulting polymers also have heads and tails. These macromolecules are polar because they are formed by head to tail condensation of polar monomers. Making Glucose: Green plants manufacture glucose through a process that requires light‚ known as photosynthesis. This process takes place in the leaf chloroplasts. Carbon dioxide and water molecules enter a sequence
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Identifying Organic Compounds Problem: Based on the color of the indicator‚ which type of organic compound will be found in each type of food? Hypothesis: If Benedict solution is added to honey‚ then it will turn. If Biuret solution is added to egg whites‚ then it will turn. If Benedict solution is added to corn oil‚ then it will turn. If Benedict solution is added to glucose‚ then it will turn. If Benedict solution is added to gelatin‚ then it will turn. If Benedict solution is added to butter
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PURPOSEThe purpose of the lab is to learn how to determine the presence of glucose‚ starch‚ lipid‚ and protein in various samples through standard tests. MODIFICATION1. Protein solution‚ vegetable oil‚ glucose solution‚ sucrose solution‚ starch solution and distilled water were added separately into six marked test tubes. The volume added was about one finger thick in depth of a test tube. 2. 5 drops of Iodine solution was added to each test tube. 3. The sample’s reaction with iodine was recorded
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