1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Salmonella enterica (#11) Gram Negative Organism | CarbohydrateFermentation(PRL) | CarbohydrateFermentation(PRG) | Indole productionTest | MethylRed Test(MR) | VogesProskauer Test | Citrate Utilization Test | Hydrogen sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella
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Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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Effectiveness of Basella rubra (Alugbati) Fruit Extract as a Counterstain in Gram Staining Investigators: Astilla‚ Yasmin Marie Cala‚ Maria Victoria Bianca Ko‚ Mariell Lizardo‚ Francis Dominic Mariano‚ Maria Cristina Querol‚ Maria Zennah Quinto‚ Camille Rosales‚ Ana Mariella Valmonte‚ Kyla Nicole Villalon‚ Gian Angelo 4MT-2 Statement of the Problem Can Basella rubra fruit extract be used as a counterstain in Gram staining? Introduction & Background According to H. J. Croxx‚ when microscopists
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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