test the purity of the unknown. After the plates were incubated and grown‚ the morphology was observed and recorded and a Gram stain was performed. After determining the Gram reaction‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables that were in the lab manual. Since the unknown #54 was determined to be a Gram positive rod‚ a thyoglycollate test was performed to determine aerobic activity. Table 1 lists the test‚ purpose‚ reagents
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Unknown Organism Report Unknown Organism # 4 Klebsiella pneumonia Klebsiella pneumonia has a rod gram stain with the agar slant cultural characteristics being slimy‚ white‚ somewhat translucent‚ raised growth. It is small gram-negative coccobacilli that can cause lobar pneumonia. The litmus milk reaction is acid‚ gas‚ and curd with a variable reaction. Klebsiella can grow either in the presence or absence of oxygen because of its nature of being a facultative anaerobe. Oxygen is a preferred energy
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Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that‚ specific biochemical tests were performed for gram positive‚ since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak‚ Blood Agar streak‚ Catalase test
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number 12 was picked. According to the procedures that we have been learned in class‚ our performances were running properly. More than 20 kinds of experiments were performed to identify unknown bacterium. The first test is gram stain. The result is pink rod which means gram negative rod shape cells. Also‚ in nutrient broth tube‚ it is observed to growth bacterium. To determine the production of protease‚ we do the gelatin stab. The result of this experiment is negative which has solid texture. The
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MOLEFHI | Identification of entrobacteriaceae | Escherichia coli cause 99% of URINARY TRACT INFECTION. | DAY 2 GRAM STAINNING Materials * Two slides * Saline water * Culture organisms * Wire loop * Gentin violet * Iodine * Safradin * 95% alcohol Procedure * Prepare slide smears of both cultured organisms and heat fix them. * Gram stain with first using Gentin violet as primary stain then‚ * Iodine as a mordent and decolourise with 95% alcohol
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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1. tested and measured? The purpose of this experiment was to determine whether the dark or light coleus leaf had the presence of starch. We used a dark and light leaf to test the presence of starch and how much was produced. 2. Summarize the results and how the results were obtained. Be sure to note the reason for each treatment of the leaves. As appropriate‚ compare experimental samples to controls. Both leaves were boiled in water then placed in hot ethyl alcohol. After adding the iodine
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The wild type and the mutant type strains of Halobacteria were used in this experiment to examine the phenotypic differences against each strain’s genotypes. The mutant strain (KBT-1) did not possess gas vesicles‚ which decreased its ability to float to the surface. The lack of gas vesicles in the mutant strain made the colonies a red color. The wild type strain (NRC-1) had gas vesicles and appeared a pink pigment color. Growth on the agar allowed one to examine the specific colonies. Inoculation
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Jody Kaur Pure Cultures Lab 1/31/13 Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate‚ which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture
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The Comparative Analysis of Simple Staining and Gram Staining Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope INTRODUCTION: A German bacteriologist‚ Dr. Theodore von Escherich‚ was the first man in 1885 who discovered the bacterium named Escherichia coli‚ which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines
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