Material and Methods * Osmosis : -thistle funnel tube -dialysis tubing sack -saturated NaCl w/food coloring -beaker with distilled H2O The experiments begins with the construction of a simple osmometer by obtaining pre-soaked length of tubing ‚opened and tied at the end. The tube is filled with saturated NaCl solution (with added food coloring) and the thistle is inserted in the dialysis tubing. The dialysis tubing is sealed to the thistle funnel with dental floss and placed in a beaker
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dialysis tubing until it softened. We then clamped on end of each dialysis tube. After this was done we filled each dialysis tube with 10 mL of a specific concentration of liquid and then clamped the other end. One with tap water‚ one with 20% sucrose‚ one with 40% sucrose‚ one with 60% sucrose and another with tap water. We then filled up the beakers with the liquid required‚ just enough so that it would cover the dialysis tubes when put in there. Beakers 1 through 4 were filled with tap water‚ while
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the swede cylinders which are put in a test tube with a low potential of sucrose solution would become turgid because the water molecules that are present in the swede will move away from an area of higher potential of water molecules to an area that has a lower potential of water molecules‚ this means that the swede sample will gain mass and become full almost to an extent where it is ready to burst. The swede samples that are going to be put in a test tube with a high potential of sucrose solution
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mixture of Carboxylic acid‚ a phenol‚ and neutral substance was placed into a reaction tube (tube 1). tert-Butyl methyl ether (2ml) was added to the tube and the solid mixture was dissolved. Next‚ 1 ml of saturated NaHCO3 solution was added to the tube and the contents were mixed separating the contents into three layers. Once this was completed the bottom layer was transferred to tube 2 using a pipette. Once in tube 2 the contents were washed with approximately 0.2 ml of ether and then the ether layer
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I predict that as the temperature is raised the number of molecules that are able to leave the cell and come into the solution will increase‚ however I think that there will be appoint at which the amount of molecules coming out will remain constant. Red beet tissue contains large amounts of betacyanin‚ a red pigment‚ located in the large internal membrane vacuoles. When the membrane is damaged‚ the pigment can cross the vacuole membrane and cell membrane. Since pigment is water soluble and not
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Gilson pipette • Eppendorf tubes • Copper sulphate • Spectrophotometer • Cuvettes • Distilled water Method: 1. Use a Gilson pipette to measure out five different solutions of copper sulphate and distilled water. 2. Pour into a 1.5ml test tube‚ label each tube 1 to 5 to indicate each solution and place the tubes into a test tube rack. Concentration of: Copper sulphate Water Test tube 1 100% 0% Test tube 2 80% 20% Test tube 3 60% 40% Test tube 4 40% 60% Test tube 5 20% 80% 3. Using an spectrophotometer
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1. Looking at the different options‚ it seems like there are only two in this scenario. Either “pull the plug” or leave it in‚ in hopes that Ricky will one day come out of the coma. If the parents decide to keep the feeding tube in place‚ Ricky will either wake up‚ or he won’t. If he does‚ he will not be a “normal” functioning adult. Just the fact of him being around could make the parents happy‚ but Ricky could be upset and unhappy with his current situation and if he is able to‚ wish his parents
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made up to 4 tubes.One tube of unknown was made by mixing 1ml of DNS with 3ml sugar solution.A blank was prepared by adding 1ml DNS to 3ml distilled water.The tubes were covered with marble and were placed in a water bath for 5 minutes.They were cooled to room temperature and then read at 540nm against the blank. Results Below is the tabulated form of the absorbance reading obtained from the spectrophotometer at 540nm: Table 1: Absorption values of the 5 tubes Tube Absorbance(nm) Concentration(
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separate test tubes 4. We labeled the tubes as #1‚ #2‚ #3 and #4 5. We added 0.1 mol dm-3 of FeCl3 into tube #2 using a pipette until a color change was observed. 6. We added 0.1 mol dm-3 of KSCN into tube #3 using a pipette until a color change was observed. 7. We added 0.1 mol dm-3 of KCl into tube #4 using a pipette until a color change was observed. 8. We did not add anything to the tube #1 because we are going to use it for comparison purposes. Qualitative Data: Tubes | 1 |
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Next‚ transferred 200 uL of the crude extract to a set of microcentrifuge tube; the blank is created by adding 200 uL of the homogenizing buffer. Then 800 uL of pre-filtered dye reagent was added to each tube and vortexed for additional 10 seconds‚ followed by an incubation period of 10 minutes (Course Supplement for Bio 101‚ p. 71). We transferred 500 uL of the solution to the cuvette and
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