1. Cut four 6-inch pieces of dialysis tubing and soak in a coffee cup filled with tap water for 2 hours prior to your start time. While waiting, prepare the sugar solutions using the following protocol: you will need a saucepan, tap water and sugar near the stove for this portion of your experiment. Prepare the following three solutions:…
Diffusion and Osmosis are two concepts that go hand in hand with each other. Diffusion is simply described as the movement of a substance from a region of high concentration to a region of low concentration. In another words, the substance will move down its concentration gradient which is “the region along which the density of a chemical substance increases or decreases” (Campbell Biology pg. 132). If you understand the concept of diffusion then osmosis is a very simple process. It can be defined as the diffusion of water across a permeable membrane. Osmosis can be cellular or artificial, so even though we are creating artificial cells in this lab, it is still considered to be osmosis. During osmosis, a solvent is trying to get through a selectively permeable membrane to make the concentration of that solvent the same on both sides of the membrane. The rate of osmosis depends on the type of environment the cell is in. There are three different environments that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment. The environment a cell is in will determine its tonicity which is “the ability of a surrounding solution to cause a cell to gain or lose…
1. The cell membrane regulates and controls what kind of molecules ______ move in & out of the cell.…
Seal the end if it is not already closed. Next, pick up a pipette and fill it with glucose. (Use this pipette only for glucose) then release the glucose into the dialysis tubing. Place four more pipettes full of glucose into the dialysis tubing. Now it is time to get another pipette (not the one used for glucose) and fill it with starch and release it into the dialysis tubing. Repeat this 4 (four) more times until there is a total of 5 pipettes of glucose and 5 pipettes of starch in the dialysis tubing. Mix the solution. Next, seal the dialysis tubing completely and make sure there is little to no air left in the dialysis tubing. Rise the bag with water. Next, fill a 400ml beaker with 300ml of water. Add 5 pipettes of Lugol´s into the 300ml of water. Mix the solution gently. Place the dialysis tubing bag into the 400ml beaker and leave for 30 minutes. After 30 minutes remove the bag and record your findings. Get 3 test tubes and label them “control” “bag” and “beaker” Next fill an unused pipette with water and pour it into the control test tube. Open the dialysis tubing and fill a pipette of the solution inside using an unused pipette. Repeat again and pour into the bag test tube. Place two pipettes of the beaker solution into the beaker tube. Add one pipettes of Benedict´s into each tube Heat all tubes for three minutes. Record…
These two Case Studies come from a National Center on Case Studies. I think that a case study approach is very useful in applying knowledge and this is what makes you learn it better. They may be a little daunting when you read them but I will help you go through them. Please ask for help so this topic becomes more enjoyable for you.…
Osmosis is the diffusion of a solvent across a selectively permeable membrane that occurs in response to differences in solute concentrations (Allen and Harper 2014).Osmosis can fall under the category of passive transport which does not require energy. With osmosis being a type of diffusion it is viewed as molecules moving from a high concentration to a low concentration. To further explain if there is a low water concentration, high amounts of solutes will be present. Water will most likely move to areas where the solute concentration is high, which demonstrates why there would be less water concentration.…
Cells have kinetic energy, a source of energy stored in cells. This energy causes molecules to bump into each other and move in new directions. Diffusion is one result of this molecular movement. Diffusion is the random movement of molecules from an area of higher concentration to areas of lower concentration. Osmosis is a special kind of diffusion where water moves through a selectively permeable membrane that only allows certain molecules to diffuse though (Lab Manual 7e, 2010).…
Both diffusion and osmosis work with the concentration gradient. In osmosis, water can travel in three different ways. If the molecules outside of the cell are lower than the concentration in the cytosol, the solution is said to by hypotonic to the cytosol, in this process, water diffuses into the cell until the cell is once again at an equilibrium. If the molecules outside of the cell are higher than the concentration in the cytosol, the solution is said to be hypertonic to the cytosol, and water diffuses out until equilibrium exists. If the molecules outside of the cell are equal then it is isotonic to the cytosol and there is no net movement of water.…
Measure and pour 50 mL of water into a 100 mL beaker. Cut a piece of dialysis tubing 15.0 cm long. Submerge the dialysis tubing in the water for at least 10 minutes. I measured and poured 82 mL water into a second 100 mL beaker. While the dialysis bag was soaking, made the glucose/sucrose mixture. Using a graduated pipette to add five mL of glucose solution to a third beaker and labeled it “Dialysis bag solution”. Used a different graduated pipette to add five mL of starch solution to the same beaker. Mixing and pipetting the solution up and down the pipette six times. Used the same pipette that I used to mix the dialysis bag solution, removing two mL of that solution and placed it in a clean beaker. That sample served as my positive control for glucose and starch. Dipped one of the glucose test strips into the two mL of glucose/starch solution in the third beaker. After one minute had passed, recorded the final color of the glucose test strip. Used a pipette to transfer approximately 0.5 mL of IKI to into the two mL of glucose/starch solution in the third beaker. After one minute had passed, recorded the final color of the glucose/starch solution. I used a clean pipette, and removed two mL of water from the 82 mL of water and placed it in a clean beaker. I dipped one of the glucose test strips into the two mL of water in the beaker. After one minute had passed, recording the final color of the glucose test strip. Used a pipette to transfer approximately 0.5 mL of IKI to into the two mL of water in the beaker. After one minute had passed, recorded the final color of the water in the beaker After at least 10 minutes had passed, remove the dialysis tube and close one end by folding over 3.0 cm of one…
Osmosis is a movement of water across a semi-permeable membrane. This may only happen when there is a difference in concentration between the two sides of the membrane. The water moves from low concentrations to high concentrations. That is because the low concentrated area tries to dilute the opposite via osmosis.…
When a substance is released into an area, the random movement of its molecules results in a multitude of collisions. These collisions, in turn, lead to a dispersion of the molecules. The overall movement of the molecules will be from an area of high concentration, where there will be more collisions, to areas of low concentration, where the number of collisions will be much less. This process of dispersion will continue until there is no net gain or loss of molecules in an area. The process by which this equilibrium occurs is called diffusion. Diffusion is vitally important to biology on many levels; individual cells, organelles, and even whole organisms rely on diffusion to carry out the processes essential to life. One especially important aspect of diffusion is osmosis, or the diffusion of water. This often occurs across a semi-permeable membrane…
* Miller, S. 2006. Animal Cells and Their Function. Pp. 126-148 in Castro, B et al. eds, Zoology (7th edition). McGraw-Hill, Texas…
For each tube, we folded up the bottom end and tied it securely so that the solution would not spill. Approximately 15mL of 0.4 sucrose solution was poured into one tube, while 15mL of 0.6 sucrose solution was poured into the other. Using our fingers, we removed most of the air out of the tube, and tied off the other end of the bag with string. Sufficient space was left so that the contents could expand. Both bags were then rinsed off gently with distilled water to remove any sucrose solution spilled. Then the outside of the bags were blotted off and the initial mass of each bag was taken, and recorded in grams. Each bag was placed in a cup that was labeled to indicate molarity of the solution in the dialysis bag. Each cup was filled with enough distilled water for the dialysis tubing to be completely submerged. The cups were left to stand for 20 minutes. At the end of this time, both bags were removed from the water, blotted and weighed again. The mass of each bag was recorded. Unlike the procedure called for in the lab manual, only 10cm of dialysis tubing was used per solution, as opposed to 30cm. Also, each cup was only filled with enough distilled water to cover the tubing, rather than 250 mL or 2/3’s of the…
I worked in a group of 4 to complete this experiment. First off I took the four sections of 15-cm long dialysis tubing that had been presoaked in dH2O and folded over one end of each tube (I found that twisting works well too) and tightly tied one of the 8 10-cm pieces of string (or waxed dental floss) that I cut with scissors around it to…
In this I will be investigating what effects the movement of water through osmosis. Osmosis is the diffusion of water. It is the process in which fluids pass through a partially-permeable membrane. It is the movement of water from high water concentration to low water concentration. Plant cells react to osmosis by hypertonic, isotonic and hypotonic.…