LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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Usman Omid Biology IB 1A The Effect of Temperature on Beetroot Membranes _Aim_ This practical will test the effect of temperature on the integrity of the membranes‚ _Introduction_ The cells of beetroot contain a pigment called betalain in their vacuoles. It is kept inside the cells by the membranes. If these membranes are damaged‚ then the betalain leaks out. The amount that leaks out can be assessed‚ as the leaked out pigment will color the water surrounding the cells. This information can
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phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate; these factors include temperature‚ pH levels‚ chemical substances and the concentration of
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Figure 3 Superoxide radical scavenging activity. Values are mean ± SEM (n = 3). ABL‚ lyophilized aqueous extract of A. bilimbi fruits; ABP‚ petroleum ether fraction of ABL; ABE‚ ethyl acetate fraction of ABL; ABB‚ butanol fraction of ABL; ABW‚ aqueous fraction of ABL; QU‚ quercetin. 3.3.5 Nitric oxide radical scavenging activity Lyophilized aqueous extract of Averrhoa bilimbi fruits (ABL) and its different solvent fractions exhibited a dose dependent inhibition of nitric oxide radicals. The IC50
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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Introduction To determine the effects of stressful experimental treatments on living membranes we are going to examine how fresh beets roots react when they are exposed to different temperatures. Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell 133). They organize specific chemicals and reactions into specific compartments within the cell. Generally‚ cell membranes consist of phospholipids
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative.
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Making an Activity Series of Metals using different Solutions Purpose/ Hypothesis: The purpose of this lab was to clearly identify the reactivity of magnesium‚ zinc and copper. The metals reactivity will be exemplified as it will be tested with different solutions such as zinc sulfate (ZnSO4)‚ copper sulfate (CuSO4)‚ hydrochloric acid (HCL)‚ magnesium sulfate (Mg2SO4)‚ iron (II) sulfate (FeSO4) and tin (II) chloride (SnCl2). This will allow us in creating an activity series to visually see the reactivity
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Paperose Lab- Introduction to Enzymes Ms. Kim/Honors Biology Purpose To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar‚ paperase and paperose. Introduction In this lab‚ your hands are the enzyme‚ paperase. This enzyme split the sugar‚ paperose‚ into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle. Materials Paperose models‚ scissors‚ plastic bag‚ container (large plastic cup)‚ stopwatch‚ Procedure 1. Each
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