The aim of this experiment is to better understand the process of fermentation of yeast in different concentrations of sucrose. The experiment worked with yeast and sugar (sucrose and glucose) to determine the rate of fermentation by testing the pressure of C02 in the test tube. The experiment tested the metabolic capability of yeast anaerobically meaning no oxygen was present (this was ensured by the thin layer of oil on the top of the solution). This means that the metabolic rate of the yeast could
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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Introduction: The purpose of this lab was to check the time needed for salivary amylase and phosphorylase to produce a negative result and how different concentrations affected those times. Enzymes are biological catalysts that can cause a specific chemical change in any part of the body (Walsh‚ 2002). Many of the reactions that take place within a cell would normally take place at temperatures substantially higher than those present inside a cell (Alberts et al.‚ 2010). Because of this‚ these chemical
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Different Concentrations and Effects The goals of this experiment were to examine the effectively of enzymes on samples of different enzyme concentrations and substrate concentrations. In addition‚ the experiment tested how effective enzymes are on samples of pH levels and temperature levels. A. Effect of Enzyme Concentration Hypothesis: With half as much enzyme concentration then the reaction rate will be half as much than when the enzyme concentration is equal to the substrate concentration
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molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it is called enzymes- substrate complex‚ and after the enzyme finish catalyzing‚ the substrates before is turned into what we called product. Enzymes‚ just like other catalyst‚ will not be used up as a part reactant through the reaction. So enzymes can be used over and over again. The substrates fit into the enzyme like a key fits into a lock (3). Enzymes have other
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Effects of Sucrose Concentration On Cell Respiration In Yeast Abstract This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration‚ so we measured the rate of cellular respiration by the amount of CO2 produced per minute. The results show a trend wherein increased concentrations of sucrose increase the rate of cellular respiration. Introduction All living cells require energy
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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Carbohydrate Substrates. Hypothesis. The hypothesis that I draw is that "" out the five carbohydrate substrates that I will use‚ Glucose will produce the highest volume of Carbon Dioxide at every five-minute interval. Null Hypothesis. The null hypothesis that I am composing is that "" the five carbohydrate substrates that I am to use will not produce any Carbon Dioxide. Scientific Research. Under anaerobic conditions (when Oxygen is unavailable) yeast cells will break down carbohydrate substrates into
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