Enzyme Activity Over Different Concentrations and Effects

Enzyme Activity Over Different Concentrations and Effects

The goals of this experiment were to examine the effectively of enzymes on samples of different enzyme concentrations and substrate concentrations. In addition, the experiment tested how effective enzymes are on samples of pH levels and temperature levels.

A. Effect of Enzyme Concentration

Hypothesis: With half as much enzyme concentration then the reaction rate will be half as much than when the enzyme concentration is equal to the substrate concentration. When the enzyme concentration is twice as much, the reaction rate will be twice the rate of the reaction of x.

Figure 1:
Time (Minutes)
Tube 2 (x)
Tube 5 (0.5x)
Tube 6 (2x)
0.5
0.037
0.013
0.076
1
0.073
0.026
0.15
1.5
0.111
0.042
0.229
2
0.15
0.059
0.306
2.5
0.192
0.077
0.383
3
0.232
0.095
0.459

Rate of Reaction for Tube 2 (x): 0.0783
Tube 5 (0.5x): 0.0331
Tube 6 (2x): 0.1538

Figure 2:

Question: When you change the enzyme to 0.5x like in tube 5, then the rate of the reaction is indeed half as much as an original enzyme reaction rate such as tube 2. The same can go for changing the enzyme to 2x (tube 6) where the reaction rate is twice as much as the original sample.

B. Effect of Substrate Concentration

Hypothesis: With half as much of hydrogen peroxide in the sample the enzyme reaction rate will be higher because there is less sample to react with and therefore it should become higher quickly. With twice as much hydrogen peroxide the enzyme reaction rate should become lower because it will take the enzyme longer now to react with the sample.

Figure 3:

Time (Minutes)
Tube 2 (x)
Tube 8 (0.5x)
Tube 9 (2x)
0.5
0.037
0.03
0.039
1
0.073
0.066
0.075
1.5
0.111
0.104
0.116
2
0.15
0.145
0.156
2.5
0.192
0.184
0.199
3
0.232
0.225
0.243

The rate of reaction for Tube 2 (x): 0.0783
Tube 8 (0.5x): 0.0783
Tube 9 (2x): 0.0818

Figure