BIOS140 W2 iLab3 Worksheet 1 
BIOS140 iLab #3: Enzyme Lab Worksheet
Name: [Insert Your Name Here]
Complete the following questions and submit your report. This document contains 4 pages. The total lab is worth 20 points.
Purpose: The purpose of this lab is to demonstrate how temperature, substrate concentration, and pH can impact the rate of an enzyme reaction.
In this experiment, you will use the Scientific Method to test your hypothesis.
The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and fructose.
Please contact me if you have any questions.
Results and Discussion:
Experiment A: Impact of Temperature on Invertase Activity
1. Propose a hypothesis to predict the effect of an increase in temperature on Inverses activity.) I propose for my hypothesis if the temperature is increases the liquids will the slopes of the chemicals average out or will the slope be greater with the temperature increase?
Experiment Results:
After you record the experimental data in EnzymeLab, record the value of the slope of each reaction below.
Experiment Run # pH Substrate concentration [S]
Temperature (°C)
Slope
(Product Concentration vs. Temp)
1
7.0
90 mM
30
.00061
2
7.0
90 mM
35
.00106
3
7.0
90 mM
40
.00147
4
7.0
90 mM
45
.00168
5
7.0
90 mM
50
.00193
6
7.0
90 mM
55
.00209
7
7.0
90 mM
60
.00168
8
7.0
90 mM
65
.00151
9
7.0
90 mM
70
.00126
10
7.0
90 mM
80
.00078
INSERT GRAPH (V0 vs. Temp) HERE:
2. Temperature with maximum initial activity (Vo): __50___ °C Based upon your hypothesis (above), is this what you expected? No, I thought that the slope would have been averaged out in the end and it seems that the 50 degrees Celsius was the peak of the experiment.
3. Do you think that Invertase isolated from any two organisms (for example, yeast Invertase vs. Invertase from the small intestine of humans) show the same temperature optimum? Why or why not? Explain your answer. I do think so as we see from the experiment the
Name: [Insert Your Name Here]
Complete the following questions and submit your report. This document contains 4 pages. The total lab is worth 20 points.
Purpose: The purpose of this lab is to demonstrate how temperature, substrate concentration, and pH can impact the rate of an enzyme reaction.
In this experiment, you will use the Scientific Method to test your hypothesis.
The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and fructose.
Please contact me if you have any questions.
Results and Discussion:
Experiment A: Impact of Temperature on Invertase Activity
1. Propose a hypothesis to predict the effect of an increase in temperature on Inverses activity.) I propose for my hypothesis if the temperature is increases the liquids will the slopes of the chemicals average out or will the slope be greater with the temperature increase?
Experiment Results:
After you record the experimental data in EnzymeLab, record the value of the slope of each reaction below.
Experiment Run # pH Substrate concentration [S]
Temperature (°C)
Slope
(Product Concentration vs. Temp)
1
7.0
90 mM
30
.00061
2
7.0
90 mM
35
.00106
3
7.0
90 mM
40
.00147
4
7.0
90 mM
45
.00168
5
7.0
90 mM
50
.00193
6
7.0
90 mM
55
.00209
7
7.0
90 mM
60
.00168
8
7.0
90 mM
65
.00151
9
7.0
90 mM
70
.00126
10
7.0
90 mM
80
.00078
INSERT GRAPH (V0 vs. Temp) HERE:
2. Temperature with maximum initial activity (Vo): __50___ °C Based upon your hypothesis (above), is this what you expected? No, I thought that the slope would have been averaged out in the end and it seems that the 50 degrees Celsius was the peak of the experiment.
3. Do you think that Invertase isolated from any two organisms (for example, yeast Invertase vs. Invertase from the small intestine of humans) show the same temperature optimum? Why or why not? Explain your answer. I do think so as we see from the experiment the