The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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emerging Rickettsial neonatal sepsis identified by PCR based DNA sequencing ABSTRACT Purpose : To detect and identify the etiological agent in the peripheral blood from the cases of neonatal sepsis. Methods: Four neonates from geographically different regions of South India presented with signs of neonatal sepsis and all the routine clinical and laboratory investigations were performed. Blood culture by Bac T Alert 3D‚ was negative. To establish the aetiology
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In The Laboratory‚ the courtesan chooses poison as her murder weapon. Poison is often the weapon of choice for female killers. It requires little or no physical strength to administer‚ and can be done secretly. It also leaves little evidence thus making it difficult to detect the culprit. We believe the act of murder is because of another woman that her lover is with and she feels physically inferior to her rival. We know this because she starts saying ‘What a drop! She’s not little‚ no minion like
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The laboratory To explore the ways aspects of power are shown in the poem. This poem is a dramatic monologue about a woman whose lover is cheating on her with his mistress ‘Pauline’. The woman who is speaking talks about her feelings of hatred and betrayal‚ so she decides to show her lover how much she is hurt by poisoning his mistress and making him watch her die slowly. Robert Browning’s poem was set in the ancient regime when women were thought of as incapable and a lower class because of their
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1. Summary In this internship‚ my company of attachment is Chevron Singapore‚ Laboratory (Lubricants Department)‚ as a Lab Analyst intern. As an intern‚ my duties are to do lab testing‚ as well as relevant paperwork involving Statistical Quality Control testing. Additionally‚ as shift work is a requirement for Lab Analysts‚ I will be helping the Analyst I am attached to for shift work in some tests such as the Analysis of Metals via Inductively Coupled Plasma Atomic Emission Spectrometry‚ and Density
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Long stands of double helical DNA can fit into the nucleus of a single cell because DNA is specially packaged through a series of compaction events to fit easily within cell nuclei. Even though the length of DNA per cell is about 100‚000 times as long as the cell itself‚ it only takes up only about 10 percent of the cell’s volume. The DNA molecule‚ in order to condense‚ wraps itself around groups of histone proteins‚ and then the chromatin folds back on it‚ nucelosomes pack together to create a compact
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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used to calculate heat transfer UA value and the effectiveness of the heat exchanger respectively. The report begins with the introduction into the experiment and gives a detailed account of the background behind heat exchangers and especially the shell and tube heat exchanger. This is followed by the list of objectives of the experiment‚ telling exactly what was expected from the laboratory and what information we wanted to gather from the experiment. The theory section explains the logic behind
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Technology based laboratory to improve science learning Abstract Till date‚ manual routine devices and methods are used to perform different test strategies in the greater part of the science research centers in our nation. The manual instruments are cumbersome though the robotized ones are expensive. It does not enthuse youthful analysts towards the science laboratories. There is a need to create applications which can be easily integrated‚ customized in school and undergrad level laboratories and are prudent
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Laboratory Report: Flame Colors of Metals Abstract The experiment about flame color of metals demonstrates how electrons jump into their excitedstate from their ground state as it came in contact with heat. As it return to its lower energy state‚it emits the absorbed energy in the form of light. A platinum or nichrome wire was dipped into aconcentrated HCL and was heated to clean it. The wire was dipped into different salt solutionsand heated in order to determine the metallic ion’s color and wavelength
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