PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
Premium DNA Molecular biology Bacteria
Movement Cardiovascular Dynamics Laboratory Report Student ID 2010004614 Due date and Value: This report must be submitted as a soft copy via email to abarnett@hku.hk no later than 5:00 pm 4 April 2012. Penalties apply for late submission‚ see course outline for details. You must attend the laboratory session to get a mark for the related report. References used when answering questions must appear in a reference list at the end of your report. Value: This report contributes 10% of your final
Premium Blood pressure
LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2.
Premium Enzyme PH
Soil Mechanics CE 32002 Oedometer Laboratory Testing Report (Consolidation) Group 13 Jordan Lowth Edwin Mwombeki Girven Venyen Division of Civil Engineering University of Dundee School of Engineering‚ Physics and Mathematics University of Dundee Table of Contents Contents 1.0 INTRODUCTION 3 2.0 AIM 4 3.0 METHODOLOGY 4 3.1 Apparatus 4 3.2 Setup 4 3.3 Procedure 5 3.4 Precautions 5 4.0 RESULTS/CALCULATIONS 6 5.0 DISCUSSION 6
Premium Soil mechanics
Experiment 6: Relative Density Laboratory Report Jessica Manansala‚ Nathaniel Martinez‚ Maria Pacia‚ Jeanelle Pedrigal‚ Miguel Poblete Department of Math and Physics College of Science‚ University of Santo Tomas España‚ Manila Philippines Abstract The experiment would introduce us to 3 concepts‚ namely‚ the definition of relative density‚ the Archimedes principle and the determination of density by the Archimedes principle. In the first activity‚ we were tasked with finding the density
Premium Density
Materials and Method In order to complete this experiment we used a combination of electrocalorimeter (immersion heater and calorimeter)‚ power supply‚ ammeter (0 to 3 A)‚ voltmeter (30 V)‚ rheostat‚ connecting wires‚ thermometer‚ stopwatch‚ laboratory balance‚ and ice. In order to obtain the data‚ the DataStudio™ software is set up on the computer to use the temperature sensor on the apparatus to collect steadily the temperatures at specified time interval of 5-seconds. After the computer is
Premium Heat Thermodynamics Energy
Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
Premium Egg DNA Cell
Quantifying the COX1 Gene within the Mitochondrial DNA of a Potato Introduction Respiration is a very important process for every living organism. While it is typically thought of as breathing in oxygen‚ and exhaling carbon dioxide‚ like all things‚ it must take place at the cellular level. The electron transport chain is responsible for cellular respiration. The process uses four complexes; the fourth is cytochrome c oxidase. Cytochrome C oxidase is responsible for the reduction of oxygen to water
Premium DNA Molecular biology
COMPARISON OF SBR AND CONTINUOUS FLOW ACTIVATED SLUDGE FOR NUTRIENT REMOVAL Alvin C. Firmin CDM Jefferson Mill‚ 670 North Commercial Street Suite 201 Manchester‚ New Hampshire 03101 ABSTRACT Sequencing batch reactors (SBR) are a flexible configuration of the activated sludge process‚ capable of providing low effluent nutrient levels. The SBR is fundamentally the same as any other variation of the activated sludge process‚ except that it operates in a batch draw and fill mode. The nuances of a
Premium Activated sludge
Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
Premium Cell Cell membrane DNA