Fritsch EF‚ Maniatis T. Molecular Cloning: a Laboratory Manual‚ 2nd ed.‚ Cold Spring Harbor Laboratory‚ Cold Spring Harbor‚ N.Y.‚ 1989. Basic Tools for DNA Manipulation and Molecular Genetic Analysis Enzymes Vectors Gel Electrophoresis PCR Southern blotting Gel Electrophoresis Gel is a complex net work of polymeric molecules Both DNA and RNA are negatively charged owing to phosphate backbone Nucleic acids migrate in a gel toward anode (+) at a rate that is inversely proportional to the log10
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October 2013 Science Informative Essay – Watson and Crick What is DNA? DNA is described as “a nucleic acid that carries the genetic information in the cell and is capable of self-reproduction and synthesis of RNA” at “The Free Dictionary”. According to nature.com‚ DNA is “a double-stranded helix‚ with the two [long chains of nucleotides] connected by hydrogen bonds.” It is the “molecule of life” and includes different amounts of the four bases (adenine
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which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin). The extracted plasmid DNA is important as it contains
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Objectives • To extract DNA and observe its characteristics Time Allocation: 1 hour‚ with overnight incubation/cooling the night before Note: Place the 91% isopropyl alcohol in the freezer the night before beginning the exercise. Note: If the mortar and pestle method is chosen‚ soak the split peas in distilled water the night before beginning the exercise. Materials |Materials |Label or Box/Bag |Qty |Item Description
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_______________________ Please see me if you have any questions 1. Which of the following is NOT a common consequence of mutations that eliminate cell-cycle checkpoints? A. polyploidy B. increased chromosomal translocations C. increased DNA damage D. aneuploidy E. Increased apoptosis 2. Attenuation is fine tuning of gene regulation of operons for enzymes associated with anabolic (chemical building) pathways‚ such as the Trp operon for tryptophan synthesis. Which of the following
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1a: Nucleic acids Our class on DNA is divided into 3 parts: (I) Genetics (II) DNA structure (III) Concepts and applications. I. Genetics: In the primordial period‚ simple molecules were formed from atoms and from these molecules‚ macromolecules were formed. These macromolecules formed life and all living organisms. The classical genetic and heredity observations in the 19th century started the search for the origin of life. The transforming principle of DNA was demonstrated from the experiment
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at the DNA sequence level. The principle behind the technology rests on the possibility of comparing band profiles generated after restriction enzyme digestion in DNA molecules of different individuals. Diverse mutations that might have occurred affect DNA molecules in different ways‚ producing fragments of variable lengths. These differences in fragment lengths can be seen after gel electrophoresis‚ hybridisation and visualisation. Isolating DNA Isolating DNA is the first step for many DNA-based
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Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. A DNA molecule which is the abbreviation for deoxyribonucleic acid is made up of precise lengthy chains of polymers and monomers and they are called nucleotides. These two (2) chains specifically which are composed of DNA strain are then molded by the grouping of the nucleotides into the polynucleotides. The nucleotide is composed of a nitrogenous base‚ a phosphate and a group. In DNA there
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Wheat Germ DNA Isolation Darius Xavier Johnson 4/4/2014 Purpose: The purpose of this experiment was to isolate the DNA of wheat germ cells and to analyze that DNA for absorption rates when denatured and annealed. Reagent Table: Reagent Molecular Weight (g/mole) Boiling Point (°C) MeltingPoint (°C) Density Hazards NaCl 58.44 1413 801 2.165 g/ml Skin and eye irritant Inhalation Ethanol 46.07 78.5 -114.1 1.59 g/ml Skin and eye irritant Introduction: Chromatin
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heat-killed S cells with living R cells. | Avery‚ McCarty‚ and MacLeod | First to show that DNA was the genetic material‚ but not believed. Repeated Griffith’s experiment‚ but subjected the strains to different enzymes‚ isolating RNA‚ DNA‚ lipids‚ carbohydrates‚ and proteins. Only the DNA killed the mice; the others had the mice survive. | Hershey and Chase | Used T4 bacteriophages in radioactive phosphorus to track DNA and radioactive sulfur to track proteins to determine what is the transformational material
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