Cooking oil Egg white solution Distilled water E Test for proteins using Albustix paper Sample Albustix paper Original colour Final colour Egg white solution Distilled water F Test for vitamin C using DCPIP solution Sample DCPIP solution Original colour Final colour Vitamin C solution Boiled vitamin C solution Distilled water Questions: 1. Do the amount of samples used in food tests need to be measured very accurately?
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determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively than others. Redox activity‚ otherwise known as oxidation-reduction
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Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change in A620 0.632 0.636 0.636 0.628 0.63 0.634 0.004 0.006 0.002 0.476 0.416 0.422 0.478 0.417 0.44 -0.002 -0.001 -0.008 Filter Colour Blue Green Red 2 DCPIP+ 5ml of sorbitol Corrected change in A620
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Abstract : The purpose of the experiment was to determine the wavelength and light intensity effect on the reaction rate of photosynthesis. Photosynthesis is the conversion of light energy to chemical energy in the form of organic compounds. There are two phases in photosynthesis‚ the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast‚ and the chloroplast contains chlorophyll pigments which absorb light energy
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generating ATP (Hoober 1984). The chemical DCPIP acts as an electron acceptor and is used to measure the rate of electron transport in the thylakoid membrane of chloroplasts (Dean and Miskiewicz 2006). Initially DCPIP is a blue colour. Although‚ when it gains electrons from the transport chain it is reduced and turns colourless (Dean and Miskiewicz 2006). A high photosynthetic rate can be interpreted by a fast rate of change from blue to colourless of the DCPIP as more electrons are flowing through the
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and ATP is done through the light reactions. Light independent reactions use carbon dioxide and the products of light reactions (ATP and NADPH) to form compounds such as glucose. The rate of photosynthesis can be determined indirect by the indicator DCPIP. When reduced this indicator changes from blue to a colorless solution. When light is absorbed‚ water is oxidized and the excited electrons are transferred for the process
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Red wavelengths will increase the rate of photosynthesis to a greater degree than blue or green wavelengths and thus will be the most optimal for plant growth. This hypothesis will be tested under laboratory conditions using silver beet extracts and DCPIP under different wavelengths and assessing the photosynthetic qualities using a spectrometer at 605nm. Photosynthesis is the process by which the suns visible light (electromagnetic radiation) is utilised by organisms with photosynthetic abilities (pigments
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fruits‚ orange‚ lemon and lime‚ and to compare them and find out which fruit contains the highest concentration of Vitamin C. By using the juice from each fruit to make a solution to combine with 10cm³ of DCPIP until the DCPIP turned colourless and recording the amount of Vitamin C needed to make the DCPIP colourless‚ the molarity of Vitamin C in each fruit could be calculated and it was found that lemon has the most amount of Vitamin C followed by lime and orange. Research Question/Hypothesis/Introduction
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Principle: Test for lipids using the grease spot test Test for glucose using Clinistix paper Test for reducing sugars using Benedict’s test Test for starch using the iodine test Test for proteins using Albustix paper Test for vitamin C using DCPIP solution A control using distilled water instead of the sample should be included. Independent: different types of food samples Dependent: different types of food substance presence in food sample Assumption: Controlled: using the same amount
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photosynthesis. The aim of this practical is to investigate the effects of different treatments on photosynthetic electron transport by using isolated chloroplasts from silverbeet leaves. As photosynthesis proceeds‚ any electrons produced will reduce the dye DCPIP to its colourless form‚ so a rapid decrease in dye colour will indicate a rapid rate of photosynthetic electron transport. Given this‚ I predict that placing the reaction in the dark will produce no change in colour as well as the treatment of adding
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