Effect of Wavelength and Light Intensity on the Reaction of Photosynthesis

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Abstract :

The purpose of the experiment was to determine the wavelength and light intensity effect on the reaction rate of photosynthesis. Photosynthesis is the conversion of light energy to chemical energy in the form of organic compounds. There are two phases in photosynthesis, the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast, and the chloroplast contains chlorophyll pigments which absorb light energy. Pigments are particular to different wavelengths. The following experiment helped determined the fastest photosynthetic reaction rate from wavelength ranging from 450, 545, 650, and 750 nm, and intensities 3, 7, 15, 35, and 150 uEinsteins/m^2/sec. The products from the light reaction are needed for the dark reaction. Therefore the light reaction rate determines the photosynthetic reaction rate. Experiment Data showed that at 650 nm and a light intensity of 35 m2/sec was a peak. The peak represented the highest points of absorbance. at those points. The absorbance determines was an optimum. Biological concepts of light absorbance support the experimental results. The wavelength at 650 nm is energetically stable and it would be able to efficiently deliver energy for the transfer of electrons. While with higher light intensities the chlorophyll readily absorbs light and photosynthetic rate increases.

This research is important because it helped determine at which wavelength of light and at which light intensity the chloroplast would generate the fastest photosynthetic reaction rate of photosynthesis. Photosynthesis takes place on chloroplasts. Chloroplasts are powered by the sun to transform light energy into chemical energy of ATP and NADPH. Photosynthesis is the biological conversion of light energy to chemical bond energy that is stored in the form of organic compounds (Falkowski, pg 1). The reaction of photosynthesis works as follows:...
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