NAME: Samantha Cruickshank ID# 620040626 Name of lab partners: Krystal Ramsaroop‚ Kristin Sigh‚ Debbion Sylvester‚ Vallene Bernard Demonstrator: Adanna DATE (of lab session): Week 7 (Tuesday 3rd‚ 2015) Course Code BIOL 1362 Title Of Lab: Estimation of Ascorbic acid Aims: To determine the mean ± S.D. ascorbic acid equivalence per 1mL of dye. To determine the ascorbic acid content in mg per mL of canned orange juice. Introduction: Vitamin C is highly water-soluble and has
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CAL Biology Practical 2 – Identification of Biochemicals in Pure Form Name :……………………………………………………………. MARK: Class :……………………………………………………………. PRACTICAL 2: IDENTIFICATION OF BIOCHEMICALS IN PURE FORM CAUTION: Any heating that has to be done in the following tests should be carried out in a water bath at the boiling point of water. Direct heating of test-tubes should not take place. Introduction Biochemical Tests Qualitative - present/ absent Semi-quantitative - present in >/<
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variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes Independent: temperature of water Dependent: % transmission of light through resulting solution The effect of changing enzyme concentration on rate of reaction. Independent: concentration of enzyme dependent:
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materials: As describe in MFHS101 biology practical guide Method: As describe in MFHS101 biology practical guide Ways to calculate Vitamin C content: Volume if standard solution to decolorize DCPIP (ml) x Amount of Vitamin C in standard solution ( 1 Volume of fruit juice sample to decolourize DCPIP (ml) mg/ml) Results: Solution | Initial volume (ml) | Final volume (ml) | Volume of juice used (ml) | Mean volume of juice
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Suggested answers to Practical Workbook for SBA Ch 1 Introducing biology Practical 1.1 Design an investigation of the effect of fresh pineapple on the setting of jelly Propose a hypothesis (p. 1-2) It is the fresh pineapple that causes the jelly to remain in liquid form. Design and perform an experiment (p. 1-2) 1 (Answer varies with Ss. The recommended quantity of jelly powder and water is stated on the packet of the jelly powder. Jelly will not set if it is too dilute.)
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of wavelengths and light intensity. Both a control and an experimental cuvette were exposed to a range of 450 to 750nm of light and varying intensities to test for reaction rates. These effects on rate were obtained by measuring the absorbance of DCPIP on a spectrophotometer after 16 minutes. We hypothesize that the least effective wavelength will be that which is reflected back at us in broad day light‚ this being green light of about 545 nm in wavelength. In regards to intensity‚ we hypothesize
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after processing is more than enough for a daily supply. DCPIP is often used as the reagent to test for vitamin C. The amount of vitamin C in a food sample can be found out by titrating with DCPIP as vitamin C can reduce blue DCPIP to colourless. However‚ it is sometimes difficult to ascertain the endpoint of the titration due to incomplete decolourisation of DCPIP. In this experiment‚ iodine and starch solutions are used instead of DCPIP to find out the amount of vitamin C in food sample. When iodine
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the reagents‚ using Gilson pipettes etc‚ study and try to identify chemical tests for food compounds. Materials and methods Reagents Monosaccharaides (glucose‚ fructose‚ maltose)‚ sucrose (disaccharide) Starch (polysaccharide)‚ egg albumin (protein) DCPIP‚ Vitamin C Olive oil + Sudan III Ethanol (highly flammable) Iodine solution (irritant) Benedicts reagents KOH + CuSO4 HCl (irritant) Honey solution Apple juice solution Milk solution Precautions Gloves Safety goggle Lab coat Gilson + tips 20 test tubes
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Section 1 These questions are about your investigation with DCPIP and measuring Vitamin C. You should use your own results‚ your graph/s and what you remember about doing your investigation to answer these questions. All answers should be in the spaces provided. 1 What were you trying to find out in your investigation? Complete the blank spaces in the sentence below. I was trying to find out if the ……………….……………………………………………….. ………………………………………………………………………...……… depends on the …………………………………………………………………………………………………
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Experiment IV: Study of Krebs cycle using Mitochondria from Mung Bean Seedlings INTRODUCTION The mitochondria is a very important organelle in the plant cell because it carries out very important cellular reactions in the cell like the Krebs cycle and oxidative phosphorylation which is how the plants produce ATP from the pyruvate produced through glycolysis (Meyer and Millar‚ 2008). Glycolysis produces a net of 2 ATP for the plant which is not enough for the cell to function while the Krebs cycle
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