another apparatus as shown in diagram 2‚ and pour 1%DCIPIP into the burette until it reaches the 50 cm3 mark. Add 1%DCIPIP from burette‚ drop by drop to the liquid broccoli and shake gently. The end point occurs when the blue colour of the final drop of DCPIP does not fade after added to the solution. Record volume used from burette in a form of a table (Table 1)‚ do this for the other three conical flasks. For the second sample of broccoli‚ instead of heating it with the Bunsen burner leave it to soak
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loss of vitamin C in fruit juices. The amount of vitamin C in the fruit juices will then be recorded to see if an increase in temperature will affect the amount of concentration of vitamin C in fruit juices. This will be determined by the amount of DCPIP used to change the colour of the fruit juice. It is expected that increasing the temperature of the fruit juices will decrease the amount vitamin C in fruit juices. Vitamin C concentration in various food substances can decrease with time based on
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employed for these purposes. In the titration of vitamin C with 2‚ 6-dichloroindophenol (DCPIP)‚ the added DCPIP will react with the vitamin C in the sample to form colorless products. During the course of the titration‚ the solution remains colorless until all the vitamin C has been reacted. A few drops of excess DCPIP will turn the solution to reddish solution. Therefore‚ DCPIP can serve as its own indicator. DCPIP decomposes with time and the decomposition products may affect the observation of end
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BIOLOGY LAB REPORT TITLE: DETERMINING THE VITAMIN C CONTENT IN VARIOUS FRUIT JUICE PREPARED BY: NOR HUSNA BINTI MOHD ROSLI CLASS: SC3 (ALUK 11) IC NUMBER: STUDENT ID: LECTURER’S NAME: MADAM ZAKIAH BINTI ZAKARIA SUBMISSION DATE: 22 AUGUST 2013 OBJECTIVES: To investigate the vitamin C content in various fruit juices which is based on the graph’s curve obtained. INTRODUCTION [pic] Vitamin C‚ or also known as ascorbic acid (L-ascorbic and L-dehydroascorbic
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pellet in 20mL 0.05M NaCl solution • Centrifuge again with same parameters • Discard liquid and resuspend pellet in 20mL of 0.5M sodium phosphate buffer at pH 6.4 Part B. The reducing power of chloroplasts • Label 9 cuvettes. Pipette 4mL and 1mL DCPIP in each. • Making blank‚ pipette 1mL chloroplast and add ascorbic acid • Set spectrophotometer to 595nm • Pipette 1mL chloroplast into each cuvette‚ make sure light is maximized • Remove foil‚ determine absorbance at time zero. Place in front of light
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water bath 95’c ‚ measuring cylinder ‚ white tile ‚ test-tube rack . Materials : Glucose‚ sucrose ‚ fructose ‚ hydrochloric acid ‚ potassium hydroxide ‚ albumin ‚ cooked starch ‚ corn oil ‚ copper (ll) sulphate solution ‚ ascorbic acid solution ‚ DCPIP solution ‚ Millon’s reagent ‚ Sudan lll ‚ tap water‚ ethanol ‚ iodine solution ‚ Benedict’s solution. Observation : Part One : Identification of Carbohydrates Reducing sugar Test Observation Fructose Colourless fructose solution turned to
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for increasing periods of time. - Method to test hypothesis: The hypothesis can be tested by first determining the amount of ascorbic acid needed to decolourize 3mL of DCPIP solution‚ and calculating the concentration of Vitamin C in the ascorbic acid. Next‚ determine the amount of fruit juice needed to decolourize 3mL of DCPIP solution‚ and calculating the concentration of Vitamin C in the fruit juice initially. Then‚ heat a few test tubes of fruit juice in a boiling water bath for different periods
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the volume of canned juice necessary to provide 75mg of Vitamin C Introduction: For the estimation of ascorbic acid‚ the method used involved the titration of the ascorbic acid‚ in the presence of a redox indicator 2‚6-dichlorophenol-indophenol (DCPIP) which acts as both an oxidant so as to oxidise the ascorbic acid to dehydroascorbic acid‚ and it acts as an indicator to determine the end point of the titration. The samples of ascorbic acid used have to be made acidic first by adding metaphosphoric
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Biology SL Internal Assessment What is the effect of heat on vitamin C concentrated in lemon juice? Introduction: “Vitamins are complex organic substances that are needed in very small amounts for many of the essential processes carried out in the body.” 1It is estimated and supported scientifically that only a few mg are filling the daily recommendation‚ which is essential for a healthy living. As most of vitamins cannot be produced by our bodies‚ we need to obtain them
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using iodine test. 4) To test the presence of lipids in corn oils when using the Sudan Ш and Emulsion tests. 5) To test the presence of proteins in an egg albumen in the Biuret’s test. 6) To test the presence of Vitamin C and ascorbic acid in the DCPIP test. Results: Figure 1 :Results of biochemical test for different food samples in test tubes In test tube A‚ the Benedict’s test is used to test for the presence of reducing sugar which is glucose.This test gives the positive result and
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