Salmonella enterica (#11) Gram Negative Organism | CarbohydrateFermentation(PRL) | CarbohydrateFermentation(PRG) | Indole productionTest | MethylRed Test(MR) | VogesProskauer Test | Citrate Utilization Test | Hydrogen sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella
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A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli An Undergraduate Research Presented to The College of Medical Technology Centro Escolar University In Partial Fulfillment Of the Requirements of the Degree Bachelor of Science in Medical Technology By: Aduana‚ Frances Mae D. Barnachea‚ Erika Joy V. Decleto‚ Pearl Yvette P. Lagmay‚ Ira Alexis C. Paris‚ Dublin Justin Patron‚ Chronielle Albert C. Radovan‚ Denzel James C
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incubated at 37C for 24- 48 hours‚ and two separate morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown
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1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal
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Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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Year Author Study Population Research Design Statistical Treatment Brief conclusion Limitation/Biases 2007 Eliana Ferreira Ozela‚ Paulo César Stringheta‚ and Milton Cano Chauca Anthocyanin in spinach vine (Basella rubra) fruits Experimental t-test In conclusion‚ the pigment extracted from the fruits of spinach vine (B. rubra) was more stable at pH 5 and pH 6. Increases in temperature and the presence of light affected the stability of this pigment. Results demonstrate that this pigment exhibits
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Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative
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MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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