function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all of these tests were performed on every culture‚ as some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation
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If there is any update‚ it will be posted no later than this Saturday (2/8/14) Identify the following individuals’ major contributions to science Ehrlich – magic bullet Hooke –cell theory and microscope Jenner - cowpox Lister- disinfectant (phenol) Koch- kochs postulate Lancefield- immunology to classify via serotypes Linnaeus- nomenclature Pasteur- pasteurization‚ vaccine‚ microbes in nonliving matter‚ aseptic techniques‚ fermentation. van Leeuwenhoek- described live organisms with Hooke’s microscope
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Observation of Bacteria on Chicken and Handrail and the Effect of GermX® and Alcohol on Them. Sujit Bhimireddy‚ Daryl Onwuchuruba‚ Naveen Sood‚ and Manasa Tripuraneni Biology 106 Abstract Bacteria are found everywhere on this planet. This experiment is aimed at observing some of the bacteria that surround us in places that we use everyday. We will look at bacteria found on a piece of chicken and bacteria found on a handrail. The effectiveness of antibacterial substances such as GermX® and
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classification of the invisible bacterial life. Bacterial classification is based on several major properties‚ CLASSIFICATION OF BACTERIA BASED ON GRAM STAINING This method of classification of bacteria was discovered by Hans Joachim Christian Gram 1883‚ a Danish Physician‚ while attempting to differentiate bacteria from human tissue by different staining methods. He noted differences in stain retention by bacteria due to
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intake of the people of Nsukka‚ Nigeria. Fish samples collected were identified and bacterial load of the samples determined using agar plate method. Differentiations and characterizations of various isolates were based on biochemical reactions and gram-staining technique. Frozen mackerel fish (Scomber scombrus) was used for the study. Invitro assay result revealed that the samples were predominantly contaminated by three bacteria species viz: Staphylococcus aureus‚ Escherichia coli and Lactobacillus plantarum
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mitosis and meiosis. 2) Differentiate gram-positive and gram-negative bacteria. a) A gram positive bacteria remains colored with gram staining even after washing with alcohol or acetone‚ whereas gram negative bacteria does not retain the stain when washed with alcohol or acetone. A gram positive bacteria outer membrane is absent and gram negative bacteria outer membrane is present. A gram positive bacteria’s peptidoglycan layer is 20-80 nm thick‚ whereas a gram negative bacteria’s cell peptidoglycan
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phagosome. This trouble-making characteristic of MTB made its identity obscured to be the diagnosed at the time when it was first discovered. This was because MTB was neither Gram negative or positive when it was Gram stained. Finally‚ the scientists employed different way of staining called acid-fast staining. Acid fast staining This technique allowed the scientists to diagnose the MTB. However‚ its treatment still remains somewhat problematic despite of its effective antibiotic treatment. This is
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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Bibliography: © The McGraw−Hill Companies‚ 2002 E X E RC I S E © The McGraw−Hill Companies‚ 2002 Gram Positive 38. General Unknown © The McGraw−Hill Companies‚ 2002
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Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours
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