colloidal components‚ tracing of DOM can be possible. Through different methods and instruments such as fluorescence excitation-emission spectroscopy‚ parallel factor analysis (PARAFAC)‚ isolation-fractionation technique (pairing of fluorescence and absorbance spectroscopy)‚ and satellite remote sensors‚ analysis of DOM can be done which can help elucidate its dynamics in aquatic environments. Introduction When a molecule absorbs light (energy)‚ an electron is excited and promoted to an unoccupied orbital
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Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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Determination of Concentration of Potassium Hydroxide Weigh boat 0.87g Weigh boat + Sulphamic acid 3.29g Weigh boat after transfer 0.87g Sulphamic acid 2.42g | Trial | 1st | 2nd | 3rd | 4th | 5th | Start | 3.00 | 3.20 | 5.20 | 18.55 | 1.90 | 14.65 | Finish | 17.35 | 18.10 | 18.10 | 31.50 | 14.65 | 27.65 | Titre | 14.35 | 14.90 | 14.90 | 12.95 | 12.75 | 13.00 | Average titre = 12.90cm3 NH2SO3H + KOH – NH2SO3K + H20 Sulphamic Acid Standard Solution Moles from mass
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of the light path * The concentration of the colored path The connection of these two variables is known as the Beer-Lambert Law: Absorbance (A) = ε c l Where ε is the absorption coefficient C is the concentration of the compound And l is the length of light usually 1cm When I is constant‚ this proves a linear relationship between absorbance and concentration. This means it can be rearranged to find the find the optimum concentration for maximum absorbance. C = A/ ε Methods:
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investigation how sugar solutions of different concentrations affect osmosis. Research Osmosis is the diffusion of water across a partially permeable membrane from a dilute solution to one of a higher concentration. If a solution has a low concentration then the amount of (in the case of my investigation) sugar‚ the solute‚ will be low and the solution will be weak. If there is high amount of sugar then the solution will be concentrated (have a high concentration). When two solutions are divided
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of electromagnetic radiation with chemical compounds (Stewart & Ebel‚ 2000). By using spectrophotometric analysis or spectrophotometry‚ one can determine the identity in terms of structure and species of a biomolecule as well as establish the concentration of a certain biomolecule (Stewart & Ebel‚ 2000). Absorption spectrophotometry is the most common of spectrophotometry technique used by biochemist. The science behind this technique is when light passes through a solution‚ components or substances
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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and the proportion of light absorbed by molecules in the medium is absorbance‚ Abs. Transmittance is defined as: T = I/Io where: Io= intensity of the incident radiation entering the medium. I = intensity of the transmitted radiation leaving the medium. T is usually expressed as percent transmittance‚ %T: %T = I/Io x 100 The Beer-Lambert law (or Beer’s law) is the linear relationship between absorbance and concentration of an absorbing species. The general Beer-Lambert law is usually
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BRADFORD ASSAY Calculation Formulas: Absorbance of BSA = A595 nm Raw Data = Average of A595 nm of three wells ÷ 3 Example: A1+A2+A3 ÷ 3 In this case‚ 0.365+0.374+0.453 ÷ 3 = .397 Corrected Data =( (A595 nm well) – BackGround) or (Raw Data – Background) Background = negative control = Bradford Reagent + No Protein Background of this standard curve = A595 nm of well ‘A’ = .397 We used well ‘A’ as our negative control. DATA RAW DATA
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Osmosis coursework Aim: To investigate the effect of different concentration of a sugar solution on the amount of osmotic activity between the solution and pear pieces of a given size. Define osmosis: Osmosis is a net movement of water/ solution molecules from a region of high concentrated area to a region of lower concentrated area through a semi-permeable membrane which lets smaller molecules such as water through but does not allot bigger molecules like glucose to pass through. The molecules
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