involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with other known concentrations‚ and then
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Abstract: The purpose of this experiment was to determine the concentration of an unknown amount of copper sulfate using the spectronic 20. Four solutions of different concentrations of H₂O and CuSO₄ were put into the spectronic 20 to observe their absorption of light. Then‚ a graph was created to plot down the measurements. Using the calibration curve of the graph‚ the concentration of the unknown substance was found out to be .12M. Background/Introduction: Beer’s law (which states that
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flask using a volumetric pipette. The volumetric flask was then filled to the mark with distilled water. The flask was covered with Parafilm and inverted to mix. The next part of the experiment was to determine the Vitamin C in a solution of known concentration. Observations of the Vitamin C sample and the amount found on the label was recorded. A tared piece of weighing paper was used to weigh 0.27 g of Vitamin C. The Vitamin C was then transferred to a clean 50 mL volumetric flask. The flask was filled
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Acid –Base Titration: A volume of 128 mL of 0.650 M Ba(OH)2 was required to completely neutralize 50.0 mL of nitric acid solution (HNO3). What is the concentration of the acid solution? Gravimetric Titration: Calculate the molar concentration of Ba2+ ions in a 500.0 mL sample of an unknown aqueous solution if 2.47 g BaSO4 is formed upon the addition of excess Na2SO4? Stoichiometry of Acid –Base Reactions: What volume of 0.800 M HCl is required to completely neutralize 35.0 g of Ca(OH)2
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1. The label CORROSIVE on a chemical container indicates Ans : ( b ) that contact destroys living tissue as well as equipment 2. FLAMMABLE means Ans : ( a ) easily ignited and capable of burning rapidly 3. " Fatal if swallowed " indicates Ans : ( c ) that the substance will cause death if ingested 4. What is the correct way to handle the following situation? Briefly explain. a. Chemical : Acetic asid i. Inhalation exposure Ans : Remove the person from the exposure. Begin rescue
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the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance at wavelength 436.0nm and 617.0 nm respectively
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Determining the Concentration of a Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more
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confirming Beer-Lambert’s Law was a successful method in determining the molar concentration of a sample within a composition that is unidentified. Absorption spectroscopy is used in order to determine the ε value; ε was calculated to be .0566. Introduction: The purpose of the Absorption Spectroscopy experiment is to evaluate the reliability and accuracy when using Beer-Lambert Law to determine the concentration of a specific sample within a mixture solution. The number of electrons being
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Osmosis Lab: Potatoes and Elodea Background Information: Cells have a need to regulate their internal environment. They need to be able to this because cells often find themselves in environments where the concentration of dissolved solids outside the cell is different from the number of dissolved solids inside the cell. Since the solids can not move across the membrane‚ the cell responds by moving water either into or out of the cell in an attempt to balance the number dissolved particles.
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