Absorption Spectroscopy Experiment: Reliability and Accuracy of Beer-Lambert Law to Determine the Sample Concentration

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Absorption Spectroscopy


Absorption spectroscopy validated Beer-Lambert’s Law, confirming Beer-Lambert’s Law was a successful method in determining the molar concentration of a sample within a composition that is unidentified. Absorption spectroscopy is used in order to determine the ε value; ε was calculated to be .0566. Introduction:

The purpose of the Absorption Spectroscopy experiment is to evaluate the reliability and accuracy when using Beer-Lambert Law to determine the concentration of a specific sample within a mixture solution.

The number of electrons being absorbed as well as the correlating photons emitted as the electron returns back down to ground state can be measured using Absorption spectroscopy. The amount of light absorption can ultimately be determined by the specific amounts of light that enter as well as the specific amounts of light that exit. Absorbance relies on the amount of molecules; the amount of molecules will increase when the number of particles also increases. The increasing of number of particles will then result in an increase in concentration. Absorption can be calculated using the Beer-Lambert Law (A = εbC; A = Absorbance, b = Path Length, and C = Concentration), the morality as well as the concentration calculations are also important parts in determining absorption. The Beer-Lambert Law constant was unidentified when performing this experiment, however the constant can be derived from the formula using the absorbance data results obtained in the absorption spectroscopy as well as the concentration values. The results of the derivation to find Beer’s constant will then be evaluated in order to validate or void the accuracy of Beer-Lambert Law to calculate absorption.

To begin this experiment; concentrated dyes were provided in order to create the different solutions as well as act as a base dye for the experiment. Serial dilution was the...
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