Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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------------------------------------------------- Liquid–liquid extraction From Wikipedia‚ the free encyclopedia Liquid–liquid extraction also known as solvent extraction and partitioning‚ is a method to separate compounds based on their relative solubilities in two different immiscible liquids‚ usually water and an organic solvent. It is an extraction of a substance from one liquid into another liquid phase. Liquid–liquid extraction is a basic technique in chemical laboratories‚ where it is performed
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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Ocean County College Department of Chemistry Liquids and Solids Date Submitted: June 21‚ 2015 Date Performed: June 19‚ 2015 Lab Section: Chem-181DL1 Course Instructor: Prof. Amal Bassa Purpose The purpose of this experiment was to determine the boiling point of isopropyl alcohol and determine the melting point of acetamide. Procedure To begin I tied the test tube and thermometer together and inserted an inverted closed-end capillary tube into the test tube. I then
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packed closely together‚ but they are able to move around. Hence a fluid does not have a rigid shape‚ but adapts to the contours of the container that holds it. Like a liquid a gas has no fixed shape‚ but it has little resistance to compression because there is enough empty space for the molecules to move closer. Whereas a liquid placed in a container will form a puddle at the bottom of the container‚ a gas will expand to fill the container. Although the three categories seem very well defined
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39. The Viscosity of Liquids After studying the present lecture‚ you will be able to Define viscosity and viscosity coefficient Outline the method to measure viscosity using Ostwald viscometer Determine the average molecular weight of a polymer Determine the surface concentration of 1-butanol in aqueous solution Measure the distribution coefficient of a solute betweenn two solvents 39.1 Introduction Viscosity‚ one of the transport properties‚ arises because of intermolecular attractive and
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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Marisol Camacho AP Chemistry Per. 1-2 10/31/12 Titration Lab Titrations are a very useful method of discovering the amount or concentration of unknown substances. The method is very well suited towards acid-base reactions. Titrations are often used in industry to analyze products to be sold. In this lab‚ standardizations will be done in the first two experiments and then titration analysis in the third. Standardization of a base (NaOH) using a primary standard (KHP) Standardization
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thermometer and placed in a Thiele tube filled with oil. The tube was heated by a Bunsen burner at approximately 1 °C per minute. Melting point temperatures were recorded at the first indication of fluid and then when the sample had turned completely liquid. Two trials were completed on each sample ². The melting point of 10% naphthalene / 90% Biphenyl mixture was determined by electronic instrumentation. Two samples were prepared and placed in closed end capillary tubes and then inserted into
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