Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Chapter 1: Introduction Chapter 1: Introduction Ionic liquids (ILs) can be viewed as a new and remarkable class of solvent. They are also a type of materials that have a long and useful history‚ where the earliest material that meets the current definition of IL was observed in the mid-19th century when a separate liquid phase called the “red oil” was observed in Friedel-Crafts reaction [17]. Over the following years‚ there has been a rapid growing of interest in ILs due to the realization
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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Part A: Diffusion on a liquid in a liquid. I did an experiment with three different glasses of tap water from the faucet. All three cups measured the same which happened to be 13 cm from the top of the cup to the bottom of the cup. I let the water settle‚ and sit for about an hour so the temperature was the same for all three cups. I then placed one drop of food coloring into the first glass and waited for the coloring to reach the bottom. I calculated 93 seconds. I then went to the second glass
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Gabriella Posess AP Chemistry- Period C 11/24/11 Iron Complex Lab Purpose: Be able to tell the difference between iron (II) and iron (III) solutions by performing redox reactions between irons oxidation states. Materials: test tube rack 6 test tubes- 25 x150 mm 25 mL graduated cylinder 6 stoppers 6 mL distilled water 6 mL Iron (III) chloride solution (0.02M FeCl3 x 6H2O) 6 mL Iron (II) sulfate solution (0.02M FeSO4 x 7H2O) 1 drop Potassium ferricyanide solution(0.1M K3Fe(CN)6)
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture‚ without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy‚ chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words‚ "chroma" meaning color
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Willy Rupprecht Date(s) performed: October 2 - 3‚ 2012 Gravimetric Analysis of an Unknown Group 1 Metal Carbonate Purpose: The purpose of this lab is to determine the identity of a Group 1 metal carbonate compound by gravimetric analysis. Procedure: The unknown carbonate is weighed and then dissolved in water. A solution of CaCl2 (calcium chloride) is added to the metal carbonate solution to precipitate the carbonate ions as CaCO3 (calcium carbonate). The precipitate is then filtered‚ dried
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